Abstract
SOMATIC cell hybridisation can reveal complementation between genetic markers1, and interspecific hybridisation provides a means to study the mechanisms involved in complementation. When the phenotypes of both parental cells are distinguishable at the molecular level, two complementation mechanisms can be recognised2. When the allele contributed by the wild type parental cell overcomes the block in the biochemical pathway characteristic of the mutant parent, products characteristic of both species should be present in the hybrid. Alternatively, if either allele acts exclusively on its own pathway, that is the wild-type allele cannot act in trans, the blockage due to the mutation remains and only the wild-type product would be expected to be synthesised in the hybrid.
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TALAVERA, A., BASILICO, C. & CROCE, C. Imperfect complementation in human-hamster somatic cell hybrids. Nature 259, 667–670 (1976). https://doi.org/10.1038/259667a0
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DOI: https://doi.org/10.1038/259667a0
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