Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

New class of enzymes acting on damaged DNA

Abstract

ONE of the major pathways of DNA repair consists of the removal and replacement of damaged nucleotides in non-replicating DNA. In the classical excision repair model1,2, the first enzymatic step in this process is the introduction of a single-strand break in the DNA adjacent to a defective nucleotide residue. Endonucleases that specifically attack DNA containing pyrimidine dinners2 or apurinic sites3–5 have subsequently been isolated from many types of cells, and enzymes of these two types have been shown to be active in DNA repair in Escherichia coli by the isolation of repair-deficient mutant strains with defective endo-nucleases6,7.

Your institute does not have access to this article

Relevant articles

Open Access articles citing this article.

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

References

  1. Howard-Flanders, P., A. Rev. Biochem., 37, 175–200 (1968).

    CAS  Article  Google Scholar 

  2. Grossman, L., in Advances in Radiation Biology, 4, (edit. by Lett, J. T., Adler, H., and Zelle, M.), 77–129 (Academic, New York, 1974).

    Google Scholar 

  3. Hadi, S. M., and Goldthwait, D. A., Biochemistry, 10, 4986–4994 (1971).

    CAS  Article  Google Scholar 

  4. Verly, W. G., Paquette, Y., and Thibodeau, L., Nature new Biol., 244, 67–69 (1973).

    CAS  Article  Google Scholar 

  5. Ljungquist, S., and Lindahl, T., J. biol. Chem., 249, 1530–1535 (1974).

    CAS  PubMed  Google Scholar 

  6. Braun, A., and Grossman, L., Proc. natn. Acad. Sci. US.A., 71, 1838–1842 (1974).

    ADS  CAS  Article  Google Scholar 

  7. Yajko, D. M., and Weiss, B., Proc. natn. Acad. Sci. U.S.A., 72, 688–692 (1975).

    ADS  CAS  Article  Google Scholar 

  8. Lindahl, T., Proc. natn. Acad. Sci. U.S.A., 71, 3649–3653 (1974).

    ADS  CAS  Article  Google Scholar 

  9. Kirtikar, D. M., and Goldthwait, D. A., Proc. natn. Acad. Sci. U.S.A., 71, 2022–2026 (1974).

    ADS  CAS  Article  Google Scholar 

  10. Jovin, T. M., Englund, P. M., and Bertsch, L. L., J. biol. Chem., 244, 2996–3008 (1969).

    CAS  PubMed  Google Scholar 

  11. Ljungquist, S., Andersson, A., and Lindahl, T., J. biol. Chem., 249, 1536–1540 (1974).

    CAS  PubMed  Google Scholar 

  12. Hadi, S. M., Kirtikar, D., and Goldthwait, D. A., Biochemistry, 12, 2747–2754 (1973).

    CAS  Article  Google Scholar 

  13. Carrier, W. L., and Setlow, R. E., Fedn. Proc., 33, 1599 (1974).

    Google Scholar 

  14. Tomita, F., and Takahashi, T., J. Virol., 15, 1073–1087 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Verly, W. G., Gossard, F., and Crine, P., Proc. natn. Acad. Sci. U.S.A., 71, 2273–2275 (1974).

    ADS  CAS  Article  Google Scholar 

  16. Ljungquist, S., Nyberg, B., and Lindahl, T., FEBS Lett., 57, 169–171 (1975).

    CAS  Article  Google Scholar 

  17. Lindahl, T., and Nyberg, B., Biochemistry, 11, 3610–3618 (1972).

    CAS  Article  Google Scholar 

  18. Lawley, P. D., and Brookes, P., Biochem. J., 89, 127–138 (1963).

    CAS  Article  Google Scholar 

  19. Markham, R., and Smith, J. D., Biochem. J., 49, 401–406 (1951).

    CAS  Article  Google Scholar 

  20. Bernardi, G., in Procedures in Nucleic Acid Research, 2, (edit. by Cantoni, G. W., and Davis, D. R.), 455–499 (Harper and Row, New York, 1971).

    Google Scholar 

  21. Friedberg, E. C., Ganesan, A. K., and Minton, K., J. Virol., 16, 315–321 (1975).

    CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

LINDAHL, T. New class of enzymes acting on damaged DNA. Nature 259, 64–66 (1976). https://doi.org/10.1038/259064a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/259064a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing