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Are glycoproteins and glycosaminoglycans components of the eukaryotic genome?

Nature volume 258, pages 639–641 (1975)Cite this article

Abstract

THE eukaryotic genome is composed primarily of DNA and a complex and heterogeneous array of proteins. Evidence is rapidly accumulating to suggest that these chromosomal proteins are important in determining structural as well as functional properties of the genome1–6. In addition to being implicated in packaging of the cell's genetic information7–12, chromosomal proteins render limited and defined genetic sequences available for transcription13–23. But the specific properties and mode of interaction of genome components remain to be resolved. We present here evidence that several molecular weight classes of chromosomal proteins are glycoproteins. Furthermore, we report the association of glycosaminoglycans, another class of carbohydrate-containing macromolecules, with the eukaryotic genome.

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References

  1. Bonner, J., et al., Science, 159, 47 (1968).

    Article  ADS  CAS  Google Scholar 

  2. Elgin, S. C., and Weintraub, H., A. Rev. Biochem., 44, 725 (1975).

    Article  CAS  Google Scholar 

  3. Hnilica, L. S., The Structure and Biological Functions of Histones, 2nd Ed. (CRC Press, Cleveland, 1975).

    Google Scholar 

  4. Stein, G. S., Spelsberg, T. C., and Kleinsmith, L. J., Science, 183, 817 (1974).

    Article  ADS  CAS  Google Scholar 

  5. Stellwagen, R. H., and Cole, R. D., A. Rev. Biochem., 38, 951 (1969).

    Article  CAS  Google Scholar 

  6. Stein, G. S., and Kleinsmith, L. J., Chromosomal Proteins and Their Role in the Regulation of Gene Expression, (Academic, New York, 1975).

    Google Scholar 

  7. Sollner-Webb, B., and Felsenfeld, G., Biochemistry, 14, 2915 (1975).

    Article  CAS  Google Scholar 

  8. Gottesfeld, J. M., Garrard, W. T., Bagi, G., Wilson, R. F., and Bonner, J., Proc. natn. Acad. Sci. U.S.A., 71, 2193 (1974).

    Article  ADS  CAS  Google Scholar 

  9. Olins, A. L., and Olins, D. E., Science, 183, 330 (1974).

    Article  ADS  CAS  Google Scholar 

  10. Hewish, D. R., and Burgoyne, L. A., Biochem. biophys. Res. Commun., 52, 504 (1973).

    Article  CAS  Google Scholar 

  11. Kornberg, R., Science, 184, 868 (1974).

    Article  ADS  CAS  Google Scholar 

  12. van Holde, K. D., Sahasrabuddhe, C. G., Shaw, B., van Brugger, E. F., and Arnberg, A. C., Biochem. biophys. Res. Commun., 60, 1365 (1974).

    Article  CAS  Google Scholar 

  13. Wang, T. Y., Expl Cell Res., 53, 288 (1968).

    Article  CAS  Google Scholar 

  14. Spelsberg, T. C., and Hnilica, L. S., Biochem. J., 120, 435 (1970).

    Article  CAS  Google Scholar 

  15. Kostraba, N. C., Montagna, R. A., and Wang, T. Y., J. biol. Chem., 250, 1548 (1975).

    CAS  PubMed  Google Scholar 

  16. Huang, R. C., and Bonner, J., Proc. natn. Acad. Sci. U.S.A., 4, 1216 (1962).

    Article  ADS  Google Scholar 

  17. Teng, C. S., Teng, C. T., and Allfrey, V. G., J. biol. Chem., 246, 3597 (1971).

    CAS  PubMed  Google Scholar 

  18. Stein, G. S., and Farber, J., Proc. natn. Acad. Sci. U.S.A., 69, 2918 (1972).

    Article  ADS  CAS  Google Scholar 

  19. Stein, G. S., Chaudhuri, S. C., and Baserga, R., J. biol. Chem., 249, 3918 (1972).

    Google Scholar 

  20. Paul, J., and Gilmour, R. S., J. molec. Biol., 34, 305 (1968).

    Article  CAS  Google Scholar 

  21. Paul, J., et al., Cold Spring Harbor Symp. quant. Biol., 38, 885 (1973).

    Google Scholar 

  22. Stein, G., Park, W., Thrall, C., Mans, R., and Stein, J., Nature, 257, 764 (1975).

    Article  ADS  CAS  Google Scholar 

  23. Kleinsmith, L. J., Heidema, J., and Carrol, A., Nature, 226, 1025 (1970).

    Article  ADS  CAS  Google Scholar 

  24. Stein, G. S., and Borun, T. W., J. Cell Biol., 52, 292 (1972).

    Article  CAS  Google Scholar 

  25. Jeffrey, P. L., and Rienits, K. G., Biochim. biophys. Acta, 141, 179 (1967).

    Article  CAS  Google Scholar 

  26. Roberts, R. M., Connor, A. B., and Cetorelli, J. J., Biochem. J., 125, 999 (1972).

    Article  Google Scholar 

  27. Brunette, D. M., and Till, J. E., J. membr. Biol., 5, 215 (1971).

    Article  CAS  Google Scholar 

  28. Aaronson, R. P., and Blobel, G., J. Cell Biol., 63, 1a (1974).

    Article  Google Scholar 

  29. Franke, W. W., Deumlang, B., Ermen, B., Jarasch, E-D., and Kleinig, H., J. Cell Biol., 46, 379 (1970).

    Article  CAS  Google Scholar 

  30. Maul, G. G., et al., J. Cell Biol., 55, 433 (1972).

    Article  CAS  Google Scholar 

  31. Aaronson, R. P., and Blobel, G., J. Cell Biol., 62, 746 (1974).

    Article  CAS  Google Scholar 

  32. Kraemer, P. M., Biochemistry, 10, 1473 (1971).

    Google Scholar 

  33. Welch, D. W., and Roberts, R. M., Pediat. Res., 9, 698 (1975).

    Article  CAS  Google Scholar 

  34. Bhavanandan, V. P., and Davidson, E. A., Proc. natn. Acad. Sci. U.S.A., 72, 2032 (1975).

    Article  ADS  CAS  Google Scholar 

  35. Krause, M. O., Kleinsmith, L. J., and Stein, G. S., Expl Cell Res., 92, 164 (1975).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Florida, Gainesville, Florida, 32610

    G. S. STEIN, R. M. ROBERTS, J. L. DAVIS, W. J. HEAD, J. L. STEIN, C. L. THRALL, J. VAN VEEN & D. W. WELCH

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  1. G. S. STEIN
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  2. R. M. ROBERTS
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  3. J. L. DAVIS
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STEIN, G., ROBERTS, R., DAVIS, J. et al. Are glycoproteins and glycosaminoglycans components of the eukaryotic genome?. Nature 258, 639–641 (1975). https://doi.org/10.1038/258639a0

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