X-ray diffraction patterns from haemoglobin-free erythrocyte membranes

Article metrics


IN a survey1 of X-ray diffraction patterns from erythrocyte membrane preparations produced by hypotonic lysis within a range of tonicities and pH, we emphasised the lability of the haemoglobin-free membrane preparation which readily degenerated during dehydration to give both lipid and residual membrane (lipoprotein) phases. The lipid was identified with a diffraction periodicity of 5–5.5 nm which was sensitive to temperature changes and which could be eliminated by treatment of the membranes with phospholipase C before dehydration2. In electron micrographs of sections prepared from the same samples the lipid phase was identified with regions of very fine layering (4 nm periodicity) scattered irregularly through a mass of closely packed layering of much greater dimension, which undoubtedly represented the condensed erythrocyte ghosts. These closely packed ghosts provided periodicities of 20–30 nm, each period including two apposed thicknesses of membrane which were clearly asymmetrical. The corresponding X-ray diffraction patterns also revealed this higher periodicity. We have since experimented with various conditions which can be used to provide haemoglobin-free membranes and have obtained much-improved X-ray diffraction data which confirm and extend our earlier conclusions.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Knutton, S., Finean, J. B., Coleman, R., and Limbrick, A. R., J. Cell Sci., 7, 357–371 (1970).

  2. 2

    Finean, J. B., and Coleman, R., FEBS Symp., 20, 9–16 (1970).

  3. 3

    Stomatoff, J. B., Krimm, S., and Harvie, N. R., Proc. natn. Acad. Sci. U.S.A., 72, 531–534 (1975).

  4. 4

    Blaurock, A. E., and Lieb, W. R., Nature, 255, 370–371 (1975).

  5. 5

    Levine, Y. K., and Wilkins, M. H. F., Nature new Biol., 230, 69–72 (1971).

  6. 6

    Finean, J. B., J. Biophys. Biochem. Cytol., 8, 13–29 (1960).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.