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Mutation blocking the specific degradation of reinitiation polypeptides in E. coli

Nature volume 257, pages 329331 (25 September 1975) | Download Citation

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Abstract

A NONSENSE mutation in a bacterial gene leads to the synthesis of two kinds of incomplete polypeptides1–4. These are the termination, or T, fragments extending from the normal N terminus to the site of mutation, and the reinitiation, or R, fragments initiating distal to the end of the T fragment and continuing to the normal C terminus. Both the T and the R fragments produced by nonsense mutations in the β-galactosidase gene of Escherichia coli (z gene) are rapidly and selectively degraded in vivo5–7. This contrasts strongly with the wild-type β-galactosidase, which is stable5,7. The degradation of mutant polypeptides implies the existence of proteolytic systems which can distinguish these unstable proteins from the stable ones.

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References

  1. 1.

    , and , J. molec. Biol., 38, 305–314 (1968).

  2. 2.

    , J. molec. Biol., 41, 329–339 (1969).

  3. 3.

    , and , J. molec. Biol., 41, 341–347 (1969).

  4. 4.

    , , and , Nature, 228, 1154–1156 (1970).

  5. 5.

    , Nature new Biol., 238, 1151–1154 (1970).

  6. 6.

    , and , J. biol. Chem., 247, 2205–2211 (1972).

  7. 7.

    , and , Proc. natn. Acad. Sci. U.S.A., 70, 2969–2973 (1973).

  8. 8.

    , and , Nature new Biol., 243, 238–241 (1973).

  9. 9.

    , et al., J. molec. Biol., 49, 251–254 (1970).

  10. 10.

    , , , and , J. molec. Biol., 12, 918–923 (1965).

  11. 11.

    , , and , C. r. hebd. Séanc. Acad. Sci. Paris, 258, 3125–3128 (1964).

  12. 12.

    , and , J. molec. Biol., 50, 359–371 (1970).

  13. 13.

    , Science, 176, 367–376 (1972).

  14. 14.

    , Nature, 227, 680–685 (1970).

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Affiliations

  1. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

    • B. N. APTE
    • , HARKER RHODES
    •  & DAVID ZIPSER

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DOI

https://doi.org/10.1038/257329a0

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