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Incorporation of GMP into specific tRNA molecules by extracts of Ehrlich ascites tumour cells

Naturevolume 257pages327329 (1975) | Download Citation



ENZYMATIC activities for synthesising four ribohomopolymers, including poly(G), have been found in rat liver, and all are stimulated by RNA1,2. Recently two poly(G) polymerases were isolated from plant cells; one requires poly(G) as a primer3 and the other denatured DNA4. We report here the isolation of an enzyme from Ehrlich ascites tumour, which catalyses the incorporation of GMP from GTP into an acid-insoluble product. This enzyme requires specific tRNAs as primers. Our data suggest that the tRNAs which can serve as primers are specific to tumours or tissues infected with tumour viruses.

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  1. Department of Molecular Biology, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan



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