Abstract
ENZYMATIC activities for synthesising four ribohomopolymers, including poly(G), have been found in rat liver, and all are stimulated by RNA1,2. Recently two poly(G) polymerases were isolated from plant cells; one requires poly(G) as a primer3 and the other denatured DNA4. We report here the isolation of an enzyme from Ehrlich ascites tumour, which catalyses the incorporation of GMP from GTP into an acid-insoluble product. This enzyme requires specific tRNAs as primers. Our data suggest that the tRNAs which can serve as primers are specific to tumours or tissues infected with tumour viruses.
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References
Wilkie, N. M., and Smellie, R. M. S., Biochem. J., 109, 485–494 (1968).
Niessing, J., and Sekeris, C. E., Nature new Biol., 243, 9–12 (1973).
Burkard, G., and Keller, E. B., Proc. natn. Acad. Sci. U.S.A., 71, 389–393 (1974).
Fukasawa, H., and Mizuochi, T., Biochem. biophys. Res. Commun., 58, 405–411 (1974).
Haruna, I., Ohno, T., Hozumi, N., and Watanabe, I., Gann. Mono. Cancer Res., 12, 203–211 (1972).
Pearson, R. L., Weiss, J. F., and Kelmers, A. D., Biochim. biophys. Acta, 228, 770–774 (1971).
Nishimura, S., in Methods in Nucleic Acid Research (edit. by Ishihama, A., Okazaki, R., Kyogoku, Y., and Nishimura, S.), 251–258 (Kyoritsu Shuppan, Tokyo, 1972).
Yamada, Y., and Ishikura, H., FEBS Lett., 29, 231–234 (1973).
Nishimura, S., in Procedures in Nucleic Acid Research (edit. by Cantoni, G. L., and Davies, D. R.), 2, 542–564 (Harper and Row, New York, Evanston, San Francisco, London, 1971).
Mikoshiba, K., Tsukada, Y., Haruna, I., and Watanabe, I., Mature, 249, 445–448 (1974).
Haruna, I., Spiegelman, S., Proc. natn. Acad. Sci. U.S.A., 54, 579–587 (1965).
Haruna, T., Ohno, T., Watanabe, I., and Ikawa, Y., Proc. Japan Acad, 46, 1016–1021 (1970).
Ohno, T., and Haruna, I., in Methods in Nucleic Acid Research (edit. by Ishihama, A., Okazaki, R., Kyogoku, Y., and Nishimura, S.), 165–168 (Kyoritsu Shuppan, Tokyo, 1972).
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ITOH, T., HARUNA, I. & WATANABE, I. Incorporation of GMP into specific tRNA molecules by extracts of Ehrlich ascites tumour cells. Nature 257, 327–329 (1975). https://doi.org/10.1038/257327a0
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DOI: https://doi.org/10.1038/257327a0
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