Letter | Published:

Release of imnumoreactive luteinising hormone-releasing hormone and thyrotrophin-releasing hormone from hypothalamic synaptosomes

Nature volume 257, pages 323325 (25 September 1975) | Download Citation

Subjects

Abstract

THE hypophysiotropic hypothalamus regulates the secretion of the anterior pituitary gland through the production and release of specific peptide hormones. Luteinising hormone-releasing hormone (LHRH) and thyrotrophin-releasing hormone (TRH) are two such peptides which have been fully characterised and synthesised1,2. Studies on intact animals have revealed the importance of various neurotransmitters and hormones in the modulation of hypothalamic LHRH and TRH secretion but little is known of the cellular mechanisms involved in such control, especially at the neurosecretory nerve terminal. We have described3,4 the use of nerve endings (synaptosomes) isolated from the mammalian hypothalamus to investigate factors which influence the release of corticotrophin-releasing factor (CRF). As this factor is uncharacterised, a major limitation of this work has been the use of bioassay techniques to measure indirectly corticotrophin-releasing activity. In the study described here radioimmunoassays have been used to measure the release of immunoreactive LHRH and TRH from synaptosomes prepared from rat and sheep hypothalami, and we report the influence of neurotransmitters and steroid hormones on these processes.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1.

    , , , , and , Biochem. biophys. Res. Commun., 43, 1344 (1971).

  2. 2.

    , , , , and , Biochem. biophys. Res. Commun., 37, 123 (1969).

  3. 3.

    , and , Nature, 251, 425 (1974).

  4. 4.

    , and , J. Endocr., 65, 33 (1975).

  5. 5.

    , , , and , Acta Endocr., 75, 625 (1974).

  6. 6.

    , , , and , J. Endocr., 59, 191 (1973).

  7. 7.

    , and , Endocrinology, 87, 249 (1970).

  8. 8.

    , and , J. Endocr., 46, 555 (1970).

  9. 9.

    , and , Endocrinology, 85, 121 (1969).

  10. 10.

    , , and , Endocrinology, 86, 278 (1969).

  11. 11.

    , and , Endocrinology, 93, 626 (1973).

Download references

Author information

Affiliations

  1. Department of Biochemistry, Imperial College, London SW7 2AY

    • G. W. BENNETT
    •  & J. A. EDWARDSON
  2. Department of Chemical Pathology, St Thomas's Hospital Medical School, London SE1, UK

    • D. HOLLAND
    • , S. L. JEFFCOATE
    •  & N. WHITE

Authors

  1. Search for G. W. BENNETT in:

  2. Search for J. A. EDWARDSON in:

  3. Search for D. HOLLAND in:

  4. Search for S. L. JEFFCOATE in:

  5. Search for N. WHITE in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/257323a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.