Letter | Published:

Cytochalasin B increases collagenase production by cells in vitro

Naturevolume 257pages243244 (1975) | Download Citation



COLLAGENASE is one of the major enzymes involved in the remodelling of connective tissues in normal and pathological states. Specific collagenases have been isolated from the culture media of many tissues in vitro1 and have been found in culture media from various cells in monolayer culture, including rabbit synovial fibroblasts2, guinea pig macrophages3,4 and human skin fibroblasts5. For a better understanding of the mechanisms by which collagenases are synthesised and released from cells, studies have focused on agents that stimulate these processes. Endotoxin3 and lymphokines4 induce release of collagenase from guinea pig macrophages, and a sustained release of collagenase and neutral protease from rabbit synovial fibroblasts was produced by phagocytosis of polystyrene latex particles6. It seems likely that all these agents affect cell membrane function, and so we have tested the effect on collagenase production by rabbit synovial fibroblasts of cytochalasin B (CB), which perturbs membranes in ways similar to phagocytosis7.

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  1. Tissue Physiology Department, Strangeways Research Laboratory, Cambridge, CB1 4RN, UK

    • E. D. HARRIS JR
    • , J. J. REYNOLDS
    •  & Z. WERB


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