Letter | Published:

Binding of aldosterone by mitochondria-rich cells of the toad urinary bladder

Naturevolume 257pages241243 (1975) | Download Citation



ALDOSTERONE causes a large increase in the active transport of sodium by the urinary bladder1 of the toad. This response, analogous to the effects of the steroid on the kidney, has been the object of considerable biochemical study2. Aldosterone also enhances the stimulation by neurohypophyseal hormones of sodium and hydro-osmotic flux3,4. Because the response in sodium transport seems quantitatively related to the increment of cyclic AMP generated by vasopressin5, augmentation of the sodium response is presumably related to the larger increment of intracellular nucleotide induced by a given dose of vaso-pressin in steroid-treated bladders6. Using a technique for separating the two major cell types of the bladder mucosal epithelium—the granular (G) and the mitochondria-rich (MR) cells—we found that a response to neurohypophyseal hormones, as reflected by hormone-induced increases in the intracellular cyclic AMP content, is apparently limited to the MR cell7. This apparent synergism of the neurohypophyseal and steroid hormones on transport suggests that their loci of action are in close proximity. We therefore examined the binding of 3H-aldosterone by the two major cell types.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.


  1. 1

    Crabbé, J., J. clin. Invest., 40, 2103–2110 (1961).

  2. 2

    Sharp, G. W. G., and Leaf, A., Physiol. Rev., 46, 593–633 (1966).

  3. 3

    Fanestil, D. D., Porter, G. A., and Edelman, I. S., Biochim. biophys. Acta, 135, 74–88 (1967).

  4. 4

    Handler, J. S., Preston, A. S., and Orloff, J., J. clin. Invest., 48, 823–833 (1969).

  5. 5

    Sapirstein, V. S., and Scott, W. N., J. clin. Invest., 52, 2379–2382 (1973).

  6. 6

    Stoff, J. S., Handler, J. S., and Orloff, J., Proc. natn. Acad. Sci. U.S.A., 69, 805–808 (1972).

  7. 7

    Scott, W. N., Sapirstein, V. S., and Yoder, M. J., Science, 184, 797–800 (1974).

  8. 8

    Ausiello, G. A., and Sharp, G. W. G., Endocrinology, 82, 1163–1169 (1969).

  9. 9

    Hunter, M. J., and Commerford, S. L., Biochim. biophys. Acta, 47, 580–586 (1961).

  10. 10

    Scott, W. N., and Sapirstein, V. S., Proc. natn. Acad. Sci. U.S.A., 72 (in the press).

  11. 11

    Voûte, C. L., Hänni, S., and Ammann, E., J. Steriod Biochem., 3, 161–165 (1972).

  12. 12

    DiBona, D. R., Civan, M. M., and Leaf, A., J. Membr. Biol., 1, 79–91 (1969).

  13. 13

    Davis, W. L., Goodman, D. B. P., Martin, J. H., Matthews, J. L., and Rasmussen, H., J. Cell Biol., 61, 554–547, (1974).

Download references

Author information


  1. Departments of Physiology and Ophthalmology, Mount Sinai School of Medicine, 100th Street and Fifth Avenue, New York, New York, 10029

    •  & WALTER N. SCOTT


  1. Search for VICTOR S. SAPIRSTEIN in:

  2. Search for WALTER N. SCOTT in:

About this article

Publication history



Issue Date



Further reading


By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.