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In vitro synthesis of tRNA precursors and their conversion to mature size tRNA

Naturevolume 257pages193197 (1975) | Download Citation

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Abstract

Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+andsu3)and tRNA2Tyr, tRNA2Gly(su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.

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Affiliations

  1. Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel

    • Violet Daniel
    • , Jacob I. Grimberg
    •  & Menachem Zeevi

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https://doi.org/10.1038/257193a0

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