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In vitro synthesis of tRNA precursors and their conversion to mature size tRNA

Abstract

Two Escherichia coli tRNA gene clusters, tRNA1Tyr (su3+andsu3)and tRNA2Tyr, tRNA2Gly(su+36), tRNA3Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.

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References

  1. Altman, S., Nature new Biol., 229, 19–21 (1971).

    Article  CAS  Google Scholar 

  2. Schedl, P., and Primakoff, P., Proc. natn. Acad. Sci. U.S.A., 70, 2091–2095 (1973).

    Article  ADS  CAS  Google Scholar 

  3. Sakano, H., Yamada, S., Ikemura, T., Shimura, Y., and Ozeki, H., Nucl. Acid Res., 1, 355–371 (1974).

    Article  CAS  Google Scholar 

  4. Burdon, R. H., Prog. Nucl. Acid Res. molec. Biol., 11, 33–73 (Academic, New York, 1971).

    Article  CAS  Google Scholar 

  5. McClain, W. H., Guthrie, C., and Barrell, B. G., Proc. natn. Acad. Sci. U.S.A., 69 3703–3707 (1972).

    Article  ADS  CAS  Google Scholar 

  6. Guthrie, C., et al., Nature new Biol., 246, 6–11 (1973).

    Article  CAS  Google Scholar 

  7. Altman, S., and Smith, J. D., Nature new Biol., 233, 35–39 (1971).

    Article  CAS  Google Scholar 

  8. Carbon, S., et al., Brookhaven Symp. Biol. (in the press).

  9. Barrell, B. G., Seidman, J. G., Guthrie, C., and McClain, W. H., Proc. natn. Acad. Sci. U.S.A., 71, 413–416 (1974).

    Article  ADS  CAS  Google Scholar 

  10. Russell, R. L., et al., J. molec. Biol., 47 1–13 (1970).

    Article  CAS  Google Scholar 

  11. Squires, C., and Carbon, J., Nature new Biol., 233 274–277 (1971).

    Article  CAS  Google Scholar 

  12. Squires, C., Konrad, B., Kirschbaum, J., and Carbon, J., Proc. natn. Acad. Sci. U.S.A., 70, 438–441 (1973).

    Article  ADS  CAS  Google Scholar 

  13. Andoh, T., and Ozeki, H., Proc. natn. Acad. Sci. U.S.A., 59 792–799 (1968).

    Article  ADS  CAS  Google Scholar 

  14. Ghysen, A., and Celis, J. E., Nature, 249, 418–421 (1974).

    Article  ADS  CAS  Google Scholar 

  15. Daniel, V., Sarid, S., Beckmann, J. S., and Littauer, U. Z., Proc. natn. Acad. Sci. U.S.A., 66, 1260–1266 (1970).

    Article  ADS  CAS  Google Scholar 

  16. Grimberg, J. I., and Daniel, V., Nature, 250, 320–323 (1974).

    Article  ADS  CAS  Google Scholar 

  17. Peacock, A. C., and Dingman, C. W., Biochemistry, 7, 668–674 (1968).

    Article  CAS  Google Scholar 

  18. Chamberlin, M., and Berg, P., Proc. natn. Acad. Sci. U.S.A., 48, 81–94 (1962).

    Article  ADS  CAS  Google Scholar 

  19. Burgess, R. R., J. biol. Chem., 244, 6160–6167 (1969).

    CAS  Google Scholar 

  20. Roberts, J. W., Nature, 244, 1168–1174 (1969).

    Article  ADS  Google Scholar 

  21. Sanger, F., Brownlee, G., and Barrell, B., J. molec. Biol., 13, 373–398 (1965).

    Article  CAS  Google Scholar 

  22. Goodman, H. M., Abelson, J., Landy, A., Brenner, S., and Smith, J. D., Nature, 217, 1019–1024 (1968).

    Article  ADS  CAS  Google Scholar 

  23. Roberts, J. W., and Carbon, J., Nature, 250, 412–414 (1974).

    Article  ADS  CAS  Google Scholar 

  24. Clarke, L., and Carbon, J., J. biol. Chem., 249, 6874–6885 (1974).

    CAS  PubMed  Google Scholar 

Download references

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Daniel, V., Grimberg, J. & Zeevi, M. In vitro synthesis of tRNA precursors and their conversion to mature size tRNA. Nature 257, 193–197 (1975). https://doi.org/10.1038/257193a0

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