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In vitro synthesis of tRNA precursors and their conversion to mature size tRNA

Nature volume 257, pages 193197 (18 September 1975) | Download Citation

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Abstract

Two Escherichia coli tRNA gene clusters, tRNA1 Tyr (su 3 +andsu 3 )and tRNA2 Tyr, tRNA2 Gly(su + 36), tRNA3 Thr, were transcribed in a purified in vitro system. Evidence indicates that the adjacent tRNA genes are transcribed together as a common precursor of large size, which, on incubation with crude cell extracts, yields mature tRNA molecules.

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Affiliations

  1. Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel

    • Violet Daniel
    • , Jacob I. Grimberg
    •  & Menachem Zeevi

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DOI

https://doi.org/10.1038/257193a0

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