Letter | Published:

In vitro recognition of carcinogen-induced local denaturation sites in native DNA by S1 endonuclease from Aspergillus oryzae

Nature 257, 151–152 (1975) | Download Citation

Subjects

Abstract

IT is known1–4 that the AAF-addition products obtained in the reaction of the ultimate carcinogen, N-acetoxy-N-2-acetylamino-fluorene (AAAF) with DNA in vitro are essentially the same as those isolated from liver DNA of rats fed with N-2-acetyl-aminofluorene (AAF). Thus, the modifications of DNA secondary structure observed in vitro should reflect DNA alterations arising in vivo. It has been shown5–9 that the main alteration induced by the fluorene ring is the creation of locally disorganised regions inside the double-helical structure. We now show that S1 endonuclease (EC3.1.4.) from Aspergillus oryzae is able to excise the local regions of denaturation induced in native DNA moleculesby AAAF.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

Rent or Buy

All prices are NET prices.

References

  1. 1

    Miller, J. A., Cancer Res., 30, 559–576 (1970).

  2. 2

    Kriek, E., Chem. biol. Interactions, 1, 3–7 (1969–70).

  3. 3

    Kriek, E., Cancer Res., 32, 2042–2048 (1972).

  4. 4

    Irving, C. C., and Veazey, R. A., Cancer Res., 29, 1799–1804 (1969).

  5. 5

    Fuchs, R., and Daune, M., Biochemistry, 11, 2659–2666 (1972).

  6. 6

    Fuchs, R., and Daune, M., FEBS Lett., 34, 295–298 (1973).

  7. 7

    Fuchs, R. P. P., and Daune, M. P., Biochemistry, 13, 4435–4440 (1974).

  8. 8

    Fuchs, R., and Daune, M., FEBS Lett., 14, 206–208 (1971).

  9. 9

    Levine, A. F., Fink, L. M., Weinstein, I. B., and Grunberger, D., Cancer Res., 34, 319–327 (1974).

  10. 10

    Miller, J. A., Sandin, R. B., Miller, E. C., and Rusch, H. P., Cancer Res., 15, 188–199 (1955).

  11. 11

    Morris, H. P., Velat, L. A., Wagner, B. P., Dahlgard, N., and Roy, F. E., J. natn. Cancer Inst., 24, 149–180 (1960).

  12. 12

    Kriek, E., Miller, J. A., Juhl, U., and Miller, E. C., Biochemistry, 6, 177–182 (1967).

  13. 13

    Miller, E. C., Juhl, U., and Miller, J. A., Science, 153, 1125–1127 (1966).

  14. 14

    Kapuler, A. M., and Michelson, A. M., Biochim. biophys. Acta, 232, 436–450 (1971).

  15. 15

    McConnel, B., and Von Hippel, P. H., J. molec. Biol., 50, 297–316 (1970).

  16. 16

    Nelson, J. H., Grunberger, D., Cantor, C. R., and Weinstein, I. B., J. molec. Biol., 62, 331–346 (1971).

  17. 17

    Troll, W., Rinde, E., and Day, P., Biochim. biophys. Acta, 174, 211–219 (1969).

  18. 18

    Weinstein, I. B., and Grunberger, D., in World Symposium on Model Studies in Chemical Carcinogenesis (edit, by Ts'o, P.O.P. and Di Paolo, J.), 217–235 (Dekker, New York, 1974).

    • 19

      Chang, C. T., Miller, J. J., and Wetmur, J. G., Biochemistry, 13, 2142–2148 (1974).

    • 20

      Weisburger, E. K., and Weisburger, J. H., Adv. Cancer Res., 5, 331–431 (1958).

    • 21

      Cerutti, P. A., Life Sci., 15, 1567–1575 (1975).

    • 22

      Vogt, V. M., Eur. J. Biochem., 33, 192–200 (1973).

    • 23

      Kay, E. R. M., Simmons, N. S., and Dounce, A. L., J. Am. chem. Soc., 74, 1724–1726 (1952).

    Download references

    Author information

    Affiliations

    1. Institut de Biologie Moleculaire et Cellulaire, 15 rue Descartes, 67000, Strasbourg, France

      • ROBERT P. P. FUCHS

    Authors

    1. Search for ROBERT P. P. FUCHS in:

    To obtain permission to re-use content from this article visit RightsLink.

    About this article

    Publication history

    Received

    Accepted

    Issue Date

    DOI

    https://doi.org/10.1038/257151a0

    Further reading

    Comments

    By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.