Letter | Published:

In vitro recognition of carcinogen-induced local denaturation sites in native DNA by S1 endonuclease from Aspergillus oryzae

Naturevolume 257pages151152 (1975) | Download Citation

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Abstract

IT is known1–4 that the AAF-addition products obtained in the reaction of the ultimate carcinogen, N-acetoxy-N-2-acetylamino-fluorene (AAAF) with DNA in vitro are essentially the same as those isolated from liver DNA of rats fed with N-2-acetyl-aminofluorene (AAF). Thus, the modifications of DNA secondary structure observed in vitro should reflect DNA alterations arising in vivo. It has been shown5–9 that the main alteration induced by the fluorene ring is the creation of locally disorganised regions inside the double-helical structure. We now show that S1 endonuclease (EC3.1.4.) from Aspergillus oryzae is able to excise the local regions of denaturation induced in native DNA moleculesby AAAF.

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  1. Institut de Biologie Moleculaire et Cellulaire, 15 rue Descartes, 67000, Strasbourg, France

    • ROBERT P. P. FUCHS

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https://doi.org/10.1038/257151a0

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