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Antisera to human B-lymphocyte membrane glycoproteins block stimulation in mixed lymphocyte culture

Nature volume 257, pages 147149 (11 September 1975) | Download Citation

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Abstract

HUMAN B cells are frequently differentiated from T cells by the ready demonstration of immunoglobulin (Ig) on their surfaces1. In the mixed lymphocyte reaction (MLR) the responding cells are mainly T cells2–4, whereas B cells have been suggested to be the primary stimulating cells4. The effect on the MLR of a specific anti-B-cell serum is therefore of considerable interest. Here we describe rabbit antisera to membrane glycoproteins of cultured human B cell lines which react only with Ig-bearing peripheral blood lymphocytes and which completely block the MLR in vitro.

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Affiliations

  1. Division of Immunology, Duke University Medical Center, Durham, North Carolina 27710

    • PETER CRESSWELL
    •  & STEVEN S. GEIER

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https://doi.org/10.1038/257147a0

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