Letter | Published:

Low doses of DNP-D-GL, a potent hapten-specific tolerogen, are immunogenic in vitro

Naturevolume 257pages141143 (1975) | Download Citation



THE DNP conjugate of a D-amino acid copolymer of glutamic acid and lysine (DNP-D-GL) is a potent inducer of DNP-specific tolerance in vivo and in vitro1,2. Simultaneous administration of DNP-D-GL and allogeneic cells to mice, however, results in anti-DNP antibody formation rather than tolerance3. DNP-D-GL thus seems to block activation of DNP-specific precursor cells in ordinary circumstances but to activate cells altered by allogeneic effect factors4. We have attempted to define further the conditions in which DNP-D-GL can induce antibody formation, a question made more relevant by the proposal that nucleic acids coupled to D-GL may be useful in preventing autoimmune disease, a situation where induction of antibody formation rather than tolerance would be unfortunate.

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  1. Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, 20014



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