Ricin resistance in baby hamster kidney cells

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LITTLE is understood about the function of the complex oligosaccharide side chains present in cell membrane glycoproteins. One approach to this problem has been to select from cultured mammalian cells variants deficient in cell surface binding sites for certain plant lectins, since in these cells some part of the membrane glycoproteins' carbohydrate moieties should be missing or altered. Such variants have been isolated from Chinese hamster ovary (CHO) cells using ricin1 (from Ricinus communis seeds) and phytohaemagglutinin2 (from Phaseolus vulgaris), cytotoxic lectins which bind to membrane glycoprotein galactose and N-acetylgalactosamine groups3–5.

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MEAGER, A., UNGKITCHANUKIT, A., NAIRN, R. et al. Ricin resistance in baby hamster kidney cells. Nature 257, 137–139 (1975) doi:10.1038/257137a0

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