Ricin resistance in baby hamster kidney cells

Article metrics

Abstract

LITTLE is understood about the function of the complex oligosaccharide side chains present in cell membrane glycoproteins. One approach to this problem has been to select from cultured mammalian cells variants deficient in cell surface binding sites for certain plant lectins, since in these cells some part of the membrane glycoproteins' carbohydrate moieties should be missing or altered. Such variants have been isolated from Chinese hamster ovary (CHO) cells using ricin1 (from Ricinus communis seeds) and phytohaemagglutinin2 (from Phaseolus vulgaris), cytotoxic lectins which bind to membrane glycoprotein galactose and N-acetylgalactosamine groups3–5.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gottlieb, C., Skinner, A. M., and Kornfeld, S., Proc. natn. Acad. Sci. U.S.A., 71, 1078–1082 (1974).

  2. 2

    Stanley, P., Caillibot, V., and Siminovitch, L., Somatic Cell Genet., 1, 1 (1975).

  3. 3

    Nicolson, G. L., and Blaustein, J., Biochim. biophvs. Acta, 266, 543–547 (1972).

  4. 4

    Nicolson, G. L., Blaustein, J., and Etzler, M., Biochemistry, 13, 196–204 (1974).

  5. 5

    Irimura, T., Kawaguchi, T., Terao, T., and Osawa, T., Carbohydrate Res., 39, 317–327 (1975).

  6. 6

    Stoker, M., and MacPherson, I., Nature, 203, 1355–1357 (1964).

  7. 7

    Nicolson, G. L., Nature, 628–630 (1974).

  8. 8

    Olsnes, S., and Pihl, A., Biochemistry, 12, 3121–3126 (1973).

  9. 9

    Carrasco, L., Fernandez-Puentes, C., and Vazquez, D., Eur. J. Biochem., 54, 499–503 (1975).

  10. 10

    Hughes, R. C., Prog. Biophys. molec. Biol., 26, 189–268 (1973).

  11. 11

    Juliano, R. L., and Stanley, P., Biochim, biophys. Acta, 389, 401–406 (1975).

  12. 12

    Gottlieb, C., Baenziger, J., and Kornfeld, S., J. biol. Chem., 250, 3303–3309 (1975).

  13. 13

    Schachter, H., et al., J. biol. Chem., 245, 1090–1100 (1970).

  14. 14

    Hynes, R. O., Cell, 1, 147–158 (1974).

  15. 15

    Smith, R. E., Zweerink, H. F., and Joklik, W. K., Virology, 39, 791–800 (1969).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

MEAGER, A., UNGKITCHANUKIT, A., NAIRN, R. et al. Ricin resistance in baby hamster kidney cells. Nature 257, 137–139 (1975) doi:10.1038/257137a0

Download citation

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.