CELL cultures of untransformed fibroblasts can be maintained in a stationary or relatively quiescent state by adjusting serum concentrations to suboptimal levels1. Addition of excess serum to quiescent cell cultures causes a rapid decrease in the intracellular concentration of cyclic AMP and a rapid increase in cyclic GMP levels2–5. These alterations in cyclic nucleotide concentrations precede the changes in macromolecular synthesis (such as RNA, protein and DNA synthesis) and growth induced by serum, and constitute one of the earliest steps in the sequence of events before the initiation of growth. The mechanism responsible for altering cyclic nucleotide concentrations in response to serum or other growth stimuli has not been elucidated however. We show here that a rapid alteration in cyclic nucleotide phosphodiesterase activity occurs when serum is added to quiescent fibroblasts and demonstrate that this alteration could mediate the changes in cyclic nucleotide levels.
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Temin, H. M., Pierson, R. W., and Dulab, N. C., in Growth, Nutrition and Metabolism of Cells in Culture (edit. by Rothblat, G. H., and Cristofals, V. J.), 50–82 (Academic London, 1972).
Sheppard, J. R., Nature new Biol., 236, 14–16 (1972).
Seifert, W., and Paul, D., Nature new Biol., 240, 281–283 (1972).
Seifert, W. E., and Rudland, P. S., Nature, 248, 138–140 (1974).
Rudland, P. S., Seeley, M., and Seifert, W., Nature, 251, 417–419 (1974).
Lineweaver, H., and Burk, D., J. Am. chem. Soc., 56, 658–666 (1934).
Schröder, J., and Plagemann, P. G. W., J. natn. Cancer Inst., 46, 423–429 (1971).
Pledger, W. J., Thompson, W. J., and Strada, S. J., J. Cyclic Nucl. Res. (in the press).
Thompson, W. J., and Appleman, M. M., Biochemistry, 10, 311–316 (1971).
Thompson, W. J., and Appleman, M. M., J. biol. Chem., 246, 3145–3150 (1971).
Steiner, A. L., Parker, C. W., and Kipnis, D. M., J. biol. Chem., 247, 1106–1113 (1972).
Thompson, W. J., and Williams, R. H., Archs biochem. Biophys., 165, 468–477 (1974).
About this article
Changes in cyclic nucleotide levels and phosphodiesterase and protein kinase activities in mitogenically stimulated 3T3 fibroblasts
Virchows Archiv B Cell Pathology Including Molecular Pathology (1981)
Induction of cell division in BALB/c-3T3 cells by phorbol myristate acetate or bovine serum: Effects of inhibitors of cyclic AMP phosphodiesterase and Na+−K+-ATPase
In Vitro (1977)