Letter | Published:

Importance of fixed sections in the study of adipose tissue cellularity

Naturevolume 256pages724725 (1975) | Download Citation



IN the study of obesity in human subjects and animal models attention has recently been directed to the size and number of cells in adipose tissue. Measurements of fat cell sizes have led to the classification of hypertrophic, hyperplastic or mixed forms of obesity in animals1 and man2. A study of the literature concerning animals, however, reveals wide discrepancies in reports of the degree of hypertrophy or hyperplasia contributing to the expansion of fat mass observed in genetically3,4 or experimentally obese animals1,5–10. These discrepancies have arisen not only because different species and strains of animals were used and different fat depots were sampled, but also because different methods were used to determine fat cell size and number.

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    Hausberger, F. X., in Handbook of Physiology. Adipose Tissue, 519–528 (Am. Physiol. Soc., Washington, DC, 1965).

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    Johnson, P. R., Zucker, L. M., Cruce, J. A. F., and Hirsch, J., J. Lipid Res., 12, 706–714 (1971).

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    Di Girolamo, M., and Mendlinger, S., Am. J. Physiol., 221, 859–864 (1971).

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  1. Clinical Research Centre, Watford Road, Harrow, Middlesex, HA1 3UJ, UK



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