Letter | Published:

Chemotaxis of lymphoblasts

Abstract

LYMPHOCYTES are motile cells whose movement in vivo seems to be directed, yet investigators over many years have repeatedly failed to show chemotaxis of lymphocytes in vitro. There have been some recent reports that lymphocytes migrate into micropore filters towards substances placed below the filter1–3, but this migration was not found to be chemotactic3. It seemed to us that our and other people's failure to demonstrate lymphocyte chemotaxis might be because we were using the wrong population of cells. The lymphocytes which can be shown most clearly to migrate into inflammatory lesions in vivo are blast-transformed cells 4–6, and we therefore examined the migration in Boyden chambers of lymphoblasts from two sources; firstly cloned human lymphoblast cell lines maintained in continuous culture7; and secondly blast cells from the lymph nodes of CBA mice, either without deliberate sensitisation with antigen, or following exposure to the contact sensitising agent, oxazolone. Both human and mouse lymphoblasts were shown to migrate into filters towards chemoattractants. The nature of this migration is discussed below.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Ward, P. A., Offen, C. D., and Montgomery, J. R., Fedn Proc., 30, 1721–1724 (1971).

  2. 2

    Higuchi, Y., Honda, M., and Hayashi, H., Cell. Immun., 15, 100–108 (1975).

  3. 3

    Schreiner, G. F., and Unanue, E. R., J. Immun., 114, 809–814 (1975).

  4. 4

    Asherson, G. L., Allwood, G. G., and Mayhew, B., Immunology, 25, 485–494 (1973).

  5. 5

    Moore, A. R., and Hall, J. G., Cell. Immun., 8, 112–119 (1973).

  6. 6

    McGregor, D. D., and Logie, P. S., J. exp. Med., 139, 1415–1430 (1974).

  7. 7

    Steel, C. M., J. natn. Cancer Inst., 48, 623–628 (1972).

  8. 8

    Wilkinson, P. C., Chemotaxis and Inflammation (Churchill-Livingstone, Edinburgh, 1974).

  9. 9

    Zigmond, S. H., and Hirsch, J. G., J. exp. Med., 137, 387–410 (1973).

  10. 10

    Bhisey, A. N., and Freed, J. J., Expl Cell Res., 64, 419–429 (1971).

  11. 11

    Allison, A. C., Davis, P., and de Petris, S., Nature new Biol., 232, 153–155 (1971).

  12. 12

    Bandmann, V., Rydgren, L., and Norberg, B., Expl Cell Res., 88, 63–73 (1974).

  13. 13

    Jensen, E. J., Korol, W., Dittmar, S. L., and Medrek, T. J., J. natn. Cancer Inst., 39, 745–754 (1967).

  14. 14

    Steel, C. M., J. natn. Cancer Inst., 47, 1193–1201 (1971).

  15. 15

    Evans, J., Steel, C. M., and Arthur, E., Cell, 3, 153–158 (1974).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

  • Chemoattractant production by synovial fluid cells in chronic arthritis. A study with a new double-chamber method for quantitating the motility of mononuclear cells labelled with chromium-51

    • P. J. Nykänen
    • , Y. T. Konttinen
    •  & V. V. Bergroth

    Clinical Rheumatology (1990)

  • Production of chemotactic factor for lymphocyte by digestion of IgG with a highly purified polymorphonuclear leukocyte neutral thiol proteinase

    • Kazuhisa Yamazaki
    • , Hisashi Abe
    • , Kohji Hara
    •  & Hiroyoshi Nohara

    Inflammation (1987)

  • Proceedings of The Irish Association for Cancer Research Meeting

    • J. P. Corridan
    • , M. A. Moran
    • , B. Herity
    • , N. Hilliard
    • , M. Moriarty
    • , J. Fennelly
    • , M. M. McCambridge
    • , M. Casey
    • , M. Quigley
    • , M. Moriarty
    • , S. Hunt
    • , Lynn Gordon
    • , W. S. Lowry
    • , M. Moriarty
    • , J. Prendiville
    • , H. Raftery
    • , P. Blake
    • , B. Herity
    • , A. Robinson
    • , R. Conroy
    • , M. Pomeroy
    • , Bernadette Herity
    • , J. F. Murphy
    • , Leslie Daly
    • , R. J. Atkinson
    • , M. Pomeroy
    • , D. Kelly
    • , M. McCabe
    • , J. J. Fennelly
    • , M. Moriarty
    • , G. Duffy
    • , F. Duff
    • , A. Robinson
    • , J. Duffy
    • , Maura Reynolds
    • , S. R. McCann
    • , P. Keeling
    • , P. J. Byrne
    • , K. Mealy
    • , A. Whelan
    • , C. Feighery
    • , T. P. J. Hennessy
    • , K. Mealy
    • , P. Keeling
    • , P. J. Byrne
    • , A. Whelan
    • , C. Feighery
    • , T. P. J. Hennessy
    • , M. J. Duffy
    • , M. O’Connell
    • , B. Cantwell
    • , J. Fennelly
    • , L. O’Siorain
    • , R. Conroy
    • , M. J. Duffy
    • , M. O’Ccnnel
    • , J. Fennelly
    • , L. O’Siorain
    • , R. Conroy
    • , J. Simon
    • , M. G. Harrington
    • , Li O’Siorain
    • , S. O’Briain
    • , S. McDonnell
    • , M. Moriarty
    • , M. Clynes
    • , C. B. Seymour
    • , C. Mothersill
    • , P. Byrne
    • , T. P. J. Hennessy
    • , M. Moriarty
    • , S. M. Lavelle
    • , M. Mac Lomhair
    • , D. O’Reilly
    • , E. Mullins
    • , S. Canty
    • , J. K. Collins
    • , J. Davis
    • , J. Conroy
    • , J. K. Collins
    • , M. B. Keatinge
    • , P. B. Collins
    • , J. P. Duignan
    • , B. Dickson
    • , A. H. Johnson
    • , A. McCann
    • , C. Seymour
    • , C. Mothersill
    • , A. Long
    • , M. Moriarty
    • , D. O’Donoghue
    • , S. Mulgrew
    • , C. Seymour
    • , C. Mothersill
    • , M. Moriarty
    • , T. J. Conere
    • , S. W. Church
    • , Sidney Lowry
    • , B. Earls
    •  & R. J. Russell

    Irish Journal of Medical Science (1985)

  • Irish association for cancer research

    • W. P. Abram
    • , F. Jones
    • , M. McGuigan
    • , N. Corbally
    • , J. F. Malone
    • , C. Mothersill
    • , C. Seymour
    • , W. M. C. Martin
    • , S. Mulgrew
    • , M. Moriarty
    • , D. Bailey
    • , J. P. Duignan
    • , B. Morrissey
    • , J. Crowe
    • , J. Lennon
    • , E. O’Malley
    • , R. J. Atkinson
    • , W. S. Lowry
    • , G. O’Loughlin
    • , N. Herity
    • , P. Blake
    • , R. Conroy
    • , K. D. Buchanan
    • , M. M. T. O’Hare
    • , T. L. Kennedy
    • , D. R. Hadden
    • , R. D. Thornes
    • , Maura Reynolds
    • , S. R. McCann
    • , Derek McCormick
    • , R. Clarke
    • , H. W. VandenBerg
    • , R. F. Murphy
    • , D. R. McCluskey
    • , R. J. Russell
    • , B. J. P. Earls
    • , H. W. VandenBerg
    • , R. Wilson
    • , D. G. Kennedy
    • , M. M. T. O’Hare
    • , I. Huda
    • , J. R. P. Gibbons
    • , T. L. Kennedy
    • , K. D. Buchanan
    • , C. F. Johnston
    • , C. Shaw
    • , K. D. Buchanan
    • , Bernadette B. Headon
    • , Denis J. Reen
    • , D. G. Kennedy
    • , H. W. VandenBerg
    • , R. F. Murphy
    • , W. S. Lowry
    • , J. Rottger
    • , D. O’Reilly
    • , L. Mullins
    • , M. McCarthy
    • , J. K. Collins
    • , C. B. Seymour
    • , Carmel Mothersill
    • , M. Moriarty
    • , J. F. Malone
    • , T. J. Conere
    • , M. G. El-Badawi
    • , O. Koriech
    • , P. W. Horton
    • , L. Gordon
    • , W. S. Lowry
    • , Ian Wallace
    •  & R. A. Meldrum

    Irish Journal of Medical Science (1984)

  • Proceedings of the Scientific Meeting held in University College, Cork on September 24th and 25th, 1982

    Irish Journal of Medical Science (1983)

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.