Letter | Published:

Independent routes for Na transport across dog red cell membranes

Naturevolume 256pages580582 (1975) | Download Citation

Subjects

Abstract

PARKER and Snow1 have shown that extracellular ATP produces an apparently nonspecific and reversible increase in the cation (Na+ and K+) permeability of dog red cells, as a result of which the cells swell gradually during suspension in an isosmolar physiological bathing medium containing ATP. This effect contrasts markedly with the usual dependence on cell volume of cation fluxes in this type of red cell2–6 which, unlike most mammalian erythrocytes, is close to ionic equilibrium with plasma6–7, and lacks an ouabain-sensitive ion pump8,9. Exogenous ATP was found to influence the surface properties of chick embryo fibroblasts10, and also cause volume changes and cation imbalances in ascites tumour cells11, and it has been suggested11 that ATP alters the passive permeability of the membrane to Na+ and K+ rather than the cation pumping mechanism per se. Effects of ATP on the membrane transport characteristics of various tissue culture cell lines have been studied12,13, and it has been proposed12 that the translocation of ATP itself may be linked with ion movement across membranes.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Parker, J. C., and Snow, R. L., Am. J. Physiol., 223, 223–888 (1972).

  2. 2

    Davson, H., J. Physiol., Lond., 101, 265 (1942).

  3. 3

    Hoffman, J. F., Am. J. Med., 41, 666 (1966).

  4. 4

    Romualdez, A., Sha'afi, R. I., Lange, Y., and Solomon, A. K. J. gen. Physiol., 60, 46 (1972).

  5. 5

    Elford, B. C., and Solomon, A. K., Biochim. biophys. Acta, 373, 253 (1974).

  6. 6

    Elford, B. C., J. Physiol., Lond., 246, 371 (1975).

  7. 7

    Berstein, R. E., Science, 120, 459 (1954).

  8. 8

    Chan, P. C., Calabrese, V., and Theil, L. S., Biochim. biophys. Acta, 79, 424 (1964).

  9. 9

    Miles, P. R., and Lee, P., J. Cell Physiol., 79, 367 (1972).

  10. 10

    Jones, B. M., Nature, 212, 362 (1966).

  11. 11

    Hempling, H. G., Stewart, C. C., and Gasic, G., J. Cell Physiol., 73, 133 (1969).

  12. 12

    Trams, E. G., Nature, 252, 480 (1974).

  13. 13

    Aiton, J. F., and Lamb, J. F., J. Physiol., Lond., 248, 14P (1975).

  14. 14

    Elford, B. C., and Solomon, A. K., Nature, 248, 522 (1974).

  15. 15

    Khan, M. M. T., and Martell, A. E., J. Am. chem. Soc., 88, 668 (1966).

  16. 16

    Caldwell, P. C., in Calcium and Cellular Function (edit. by Cuthbert, A. W.), (Macmillan, London, 1970).

  17. 17

    Davson, H., and Reiner, J. M., J. cell comp. Physiol., 20, 325 (1942).

  18. 18

    Sha'afi, R., and Pascoe, E., J. gen Physiol., 61, 709 (1973).

  19. 19

    Hille, B., Progr. Biophys., 21, 3 (1970).

Download references

Author information

Affiliations

  1. Division of Cryobiology, Clinical Research Centre, Harrow, HA1 3UJ, UK

    • B. C. ELFORD

Authors

  1. Search for B. C. ELFORD in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/256580a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.