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Reversible arrest of mouse 3T6 cells in G2 phase of growth by manipulation of a membrane-mediated G2 function

Naturevolume 256pages578580 (1975) | Download Citation

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Abstract

BESIDES replicating their genetic material and subsequently partitioning it between two daughters, cells of higher organisms also generally contain two distinct periods during their growth cycle in which they are engaged in neither activity. The first of these is a gap which occurs between the completion of one division and the onset of the next round of DNA synthesis, and is abbreviated as G1. The ability to obtain stable populations of cells reversibly arrested in G1 (refs 1–3) has made possible extensive investigation of this stage of the cell cycle4. The second gap (G2) lies between the completion of DNA synthesis and the onset of mitosis. Chiefly because of the unavailability of techniques for reversibly holding cells at this stage, the possible functions of G2 have remained largely unprobed5. I now report a method by which reversibly-arrested G2 cells can be obtained and tentatively suggest one function of this phase as being the reorganisation of the cell surface in preparation for the start of mitosis.

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References

  1. 1

    Todaro, G. J., Lazar, G. K., and Green, H., J. cell. comp. Physiol., 66, 325–334 (1965).

  2. 2

    Shodell, M., and Rubin, H., In Vitro, 6, 66–74 (1970).

  3. 3

    Burk, R. R., J. exp. Cell Res., 63, 309–316 (1970).

  4. 4

    Padilla, G. M., Cameron, I. L., and Zimmerman, A., Cell Cycle Controls (Academic, New York and London, 1974).

  5. 5

    Tobey, R. A., Nature, 254, 245–247 (1975).

  6. 6

    Nicolson, G. L., Int. Rev. Cytol., 39, 90–190 (1974).

  7. 7

    Edelman, G. M., Yahara, I., and Wang, J. L., Proc. natn. Acad. Sci. U.S.A., 70, 1442–1446 (1973).

  8. 8

    Edidin, M., in Proc. ninth Can. Cancer Res. Conf. (edit. by Scholefield, P. G.), 95–113 (University of Toronto Press, 1972).

  9. 9

    Singer, S. J., Adv. Immun., 19, 1–66 (1974).

  10. 10

    Edidin, M., and Weiss, A., Proc. natn. Acad. Sci. U.S.A., 69, 2456–2459 (1972).

  11. 11

    Horwitz, A. F., Hatten, M. E., and Burger, M. M., Proc. natn. Acad. Sci. U.S.A., 71, 3115–3119 (1974).

  12. 12

    Sachs, L., Inbar, M., and Shinitzky, M., in Control of Proliferation in Animal Cells (edit. by Clarkson, B., and Baserga, R.), 283–296 (Cold Spring Harbor Press, New York, 1974).

  13. 13

    Rittenhouse, H. G., Williams, R. E., Wisnieski, B., and Fox, C. F., Biochem. biophys. Res. Commun., 58, 222–228 (1974).

  14. 14

    Steim, J. M., FEBS Symposia, 28, 185–196 (1972).

  15. 15

    Wisnieski, B. J., Parkes, J. G., Huang, Y. O., and Fox, C. F., Proc. natn. Acad. Sci. U.S.A., 71, 4381–4385 (1974).

  16. 16

    Lyons, J. M., Cryobiology, 9, 341–350 (1972).

  17. 17

    Phillips, M. C., in Progress in Surface and Membrane Science (edit. by Danielli, J. F., and Rosenberg, M. D.), 139–221 (Academic, New York and London, 1972).

  18. 18

    Chapman, D., in Biomembranes, 4 A, 123–158 (Plenum, New York, 1974).

  19. 19

    Williams, R. E., Li, J. K.-K., and Fox, C. F., Biochem. biophys. Res. Commun., 62, 462–469 (1975).

  20. 20

    Raff, M. C., de Petris, S., and Mallucci, L., in Control of Proliferation in Animal Cells (edit. by Clarkson, B., and Baserga, R.), 271–281 (Cold Spring Harbor Press, New York, 1974).

  21. 21

    Smets, L. A., and Ley, L., J. Cell Physiol., 84, 343–348 (1974).

  22. 22

    Watanabe, I., and Okada, S., J. Cell Biol., 32, 309–323 (1967).

  23. 23

    Rao, P. N., and Engelberg, J., in Cell Synchrony (edit by Cameron, I. L., and Padilla, G. M.), 332–253 (Academic, New York and London, 1966).

  24. 24

    Amos, H., Hoyt, R. F., and Horisberger, M., In Vitro, 6, 190–196 (1970).

  25. 25

    Tobey, R. A., Crissman, H. A., and Kraemer, P. M., J. Cell Biol., 54, 638–645 (1972).

  26. 26

    Folch, J., Lees, M., and Sloan-Stanley, G. H., J. biol. Chem., 226, 497–609 (1957).

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  1. Imperial Cancer Research Fund, PO Box 123, Lincoln's Inn Fields, London, WC2A 3PX, UK

    • MIKE SHODELL

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https://doi.org/10.1038/256578a0

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