Letter | Published:

Tryptic destruction of aggregative competence in Dictyostelium discoideum and subsequent recovery

Abstract

WHEN they reach the stationary phase, the vegetative cells of Dictyostelium discoideum become adhesive and form multicellular aggregates before forming fruiting bodies. In shaken liquid suspension they form aggregates in two stages1. First, they produce loose amorphous clusters which, if deposited on a solid substratum, immediately disperse. In the second stage (6–7 h) the clusters have become tight, round or ovoid aggregates eventually covered with a thin cortical sheath. If deposited on solid substratum, they remain intact and immediately proceed to the next stage of fruiting body construction. The loose clusters are EDTA-sensitive, the tight aggregates are not2.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gerisch, G., Current Topics in Developmental Biology (edit. by Moscona, A., and Monroy, A.) (Academic, New York, 1968).

  2. 2

    Beug, H., Katz, F. E., and Gerisch, G., J. Cell Biol., 56, 647 (1973).

  3. 3

    Sussman, M., and Boschwitz, C., Devl Biol. (in the press).

  4. 4

    Sonneborn, D. R., Sussman, M., and Levine, L., J. Bact., 87, 1321 (1964).

  5. 5

    Beug, H., Gerisch, G., Kempff, S., Riedel, V., and Cremer, G., Expl Cell Res., 63, 147 (1970).

  6. 6

    Newell, P. C., Longlands, M., and Sussman, M., J. molec. Biol., 58, 541 (1971).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.