WHEN DNA is treated with ultraviolet light (220–300 nm) cyclobutyl pyrimidine dimers are produced between adjacent pyrimidines on a strand of DNA1. These dimers are a major cause of mutations and death in simple organisms after ultraviolet-irradiation1, and they have been implicated in the induction of skin cancer in man2,3. An important tool for assessing the deleterious effects of dimers is the photoreactivation test: the photoreactivating enzyme repairs DNA by the specific and exclusive monomerisation of dimers in a light-dependent (> 300nm) reaction4,5. If ultraviolet biological damage can be reversed by true photoenzymatic repair, then dimers have a major role in the production of that damage6. For the test to assess the role of ultraviolet-induced dimers in human carcinogenesis at least three criteria must be met: (1) human cells must possess a functional photoreactivating enzyme, (2) this enzyme must be able to monomerise dimers in DNA, and (3) the enzyme must be able to restore biological activity to ultraviolet-irradiated DNA. It has been shown that human cells meet the first two criteria7,8; we now show that human photoreactivating enzyme in fibroblasts can restore infectivity to ultraviolet-irradiated herpes simplex virus (HSV).
Access optionsAccess options
Subscribe to Journal
Get full journal access for 1 year
only $3.90 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Setlow, R. B., Science, 153, 379–386 (1966).
Epstein, J. H., in Photophysiology, 5, (edit. by Giese, A. C.) 235–273 (Academic, New York, 1971).
Robbins, J. H., Lutzner, M. A., Festoff, B. W., and Coon, H. G., Ann. internal Med., 80, 221–248 (1974).
Rupert, C. S., J. gen. Physiol., 43, 573–595 (1962).
Setlow, J. K., and Setlow, R. B., Nature, 197, 560–562 (1963).
Cook, J. S., in Photophysiology (edit. by Giese, A. C.), 3, 191–233 (Academic, New York, 1971).
Sutherland, B. M., Nature, 248, 109–112 (1974).
Sutherland, B. M., Rice, M., and Wagner, E. K., Proc. natn. Acad. Sci. U.S.A., 72, 103–107 (1975).
Wagner, E., Swanstrom, R., and Stafford, M., J. Virol., 10, 675–682 (1972).
Harm, W., Rupert, C. S., and Harm, H., in Molecular and cellular repair processes (edit. by Beers, R. F., Herriott, R. M., and Tilghman, R. C.), 53–63 (Johns Hopkins University Press, Baltimore, 1971).
Rabson, A. S., Tyrrell, S. A., Legallais, F. Y., Proc. Soc. Exp. Biol. Med., 132, 802–806 (1969).
Lytle, C. D., Aaronson, S. A., and Harvey, E., Int. J. Radiat. Biol., 22, 159–165 (1972).
Jagger, J., Introduction to Research in Ultraviolet Photobiology (Prentice Hall, Englewood Cliffs, New Jersey, 1967).
Sutherland, B. M., and Chamberlin, M. J., Analyt. Biochem., 53, 168–176 (1973).
Lowry, O. H., Rosebrough, M. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265–275 (1951).
About this article
Photoreactivation and ultraviolet-enhanced reactivation of ultraviolet-irradiated nuclear polyhedrosis virus by insect cells
Archives of Virology (1984)
Archives of Virology (1979)