Letter | Published:

Immunological and bacteriological basis for vaccination against dental caries in rhesus monkeys

Abstract

A REPRODUCIBLE model for dental caries has been established in rhesus monkeys1. The development of caries is comparable to that found in man because caries is induced by maintaining the animals on a human type of diet, this is associated with an overgrowth of a naturally acquired Streptococcus mutans and caries develops in teeth that are morphologically similar to human teeth. The incidence of smooth surface caries was reduced by subcutaneous or submucous immunisation with a heat-killed Strep. mutans in Freund's incomplete adjuvant (FIA)2. Protection has been correlated predominantly with the rate of development of serum complement fixing antibodies to a hydroxyl apatite fraction of the culture supernatant (HACS) of Strep. mutans. The aims of this investigation were to attempt to elucidate the mechanism of prevention of dental caries in rhesus monkeys by immunisaion with a monkey passaged Strep. mutans. The relative effects of saliva and crevicular fluid on Strep. mutans were tested by developing a differential sampling technique across the plaque and the adjacent saliva and crevicular fluid. This revealed that the very low caries score in the immunised animals was correlated with serum antibodies and with a reduction in the number of Strep. mutans in crevicular fluid and the adjacent bacterial plaque zone.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Lehner, T., Challacombe, S. J., and Caldwell, J., Archs oral Biol. (in the press).

  2. 2

    Lehner, T., Challacombe, S. J., and Caldwell, J., Archs oral Biol. (in the press).

  3. 3

    Hurme, V. O., Ann. N. Y. Acad. Sci., 85, 795 (1960).

  4. 4

    Challacombe, S. J., Guggenheim, B., and Lehner, T., Archs oral Biol., 18, 657 (1973).

  5. 5

    de Stoppelaar, J. D., van Houte, J., and Backer Dirks, O., Caries Res., 3, 190 (1969).

  6. 6

    Skapski, H., and Lehner, T., Int. Assoc. dent. Res. (Abstract, in the press).

  7. 7

    Genco, R. J., Evans, A. T., and Taubman, M. A., Adv. exp. Med. Biol., 45, 327 (1974).

  8. 8

    Bowen, W. H., Br. dent. J., 126, 159 (1969).

  9. 9

    Tanzer, J. M., Hageage, C. J., Jr., and Larson, R. H., Archs oral Biol., 18, 1425 (1973).

  10. 10

    Taubman, M. A., and Smith, D. J. Infect. Immun., 9, 1079 (1974).

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.