Letter | Published:

Non-repetitive DNA transcripts in nuclei and polysomes of polyoma-transformed and non-transformed mouse cells

Naturevolume 254pages356358 (1975) | Download Citation



WE have determined the extent to which non-repetitive DNA is represented in nuclear and polysomal RNA of polyoma-transformed and non-transformed mouse cells in culture. Our RNA–DNA saturation-hybridisation experiments have shown that in transformed cells, polysomes contain only 10–20% of the informational complexity present in nuclear RNA. Similar results were obtained with RNA from confluent cultures of non-transformed cells. In subconfluent cultures of non-transformed cells, however, polysomes contained about 40% of the sequence diversity present in nuclear RNA. Further experiments, with appropriate RNA mixtures, suggest that there are also qualitative differences in polysomal RNA between transformed and non-transformed cells, as well as between confluent and subconfluent cultures of non-transformed cells.

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  1. Division of Laboratories and Research, New York State Department of Health, Albany, New York, 12201

    • L. J. GRADY
    •  & W. P. CAMPBELL


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