Abstract
SPECIFIC proteolytic enzymes have been used to dissociate adult vertebrate retinae into single cells1–3. These methods facilitate the study of chemical and physiological properties of isolated, identified retinal cells. In particular, the synthesis of neurotransmitter candidates in a pure retinal cell type has been studied by drawing identified cells into a micropipette, incubating them with appropriate radioactive precursors, and analysing the presence of radioactive transmitters1. In the study reported here, this method was used to examine transmitter synthesis by horizontal cells. Cajal's internal horizontal cells4 from the goldfish retina were used because they could be dissociated easily from the retina and identified readily. In addition, these cells have been shown to be connected to Cajal's external horizontal cells, and can therefore be considered as fusiform axon terminals of cone horizontal cells5,6.
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LAM, D. Biosynthesis of γ-aminobutyric acid by isolated axons of cone horizontal cells in the goldfish retina. Nature 254, 345–347 (1975). https://doi.org/10.1038/254345a0
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DOI: https://doi.org/10.1038/254345a0
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