Letter | Published:

Conservation of primary structure in 16S ribosomal RNA

Naturevolume 254pages8386 (1975) | Download Citation

Subjects

Abstract

THERE is no doubt that the large ribosomal RNAs play specific roles in ribosome function1. Yet the thrust of experimentation during the past 5 years indicates clearly that the biologist tends to view these roles as structural, function being reserved by and large for the ribosomal protein components2,3. Whether or not this prejudice can be maintained remains to be seen. In any case, the existence of an approximate sequence for a 16S rRNA provides a basis for detailed characterisations of this molecule3,4. Here we begin to define 16S rRNA function by localising the major conserved regions in the molecule through a comparative analysis of 27 prokaryotic 16S rRNA primary structures.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Traub, P., and Nomura, M., Proc. natn. Acad. Sci. U.S.A., 59, 777–784 (1968).

  2. 2

    Garrett, R. A., and Wittmann, H. G., Adv. Protein Chem., 27, 277–347 (1973).

  3. 3

    Fellner, P., Ehresmann, C., Stiegler, P., and Ebel, J.-P., Nature new Biol., 239, 1–5 (1972)

  4. 4

    Ehresmann, C., Stiegler, T., Mackie, G., Zimmerrmann, R. A., Ebel, J. -P., and Fellner, P., in Handbook of nucleic acid sequences, 73 (Joynson-Bruvvers, Oxford 1974).

  5. 5

    Pechman, K. J., and Woese, C. R., J. molec. Evol., 1, 230–240 (1972).

  6. 6

    Sogin, S. J., Sogin, M. L., and Woese, C. R., J. molec. Evol., 1, 173–184 (1972).

  7. 7

    Uchida, T., Bonen, L., Schaup, H. W., Lewis, B. J., Zablen, L., and Woese, C. R., J. molec. Evol., 3, 63–77 (1974).

  8. 8

    Woese, C. R., Sogin, M. L., and Sutton, L. A., J. molec. Evol., 3, 293–299 (1974).

  9. 9

    Zablen, L., Bonen, L., Meyer, R., and Woese, C., J. molec. Evol. (in the press).

  10. 10

    Zablen, L. thesis, Univ. Illinois (1975).

  11. 11

    Doolittle, W. F., Woese, C. R., Sogin, M. L., Bonen, L., and Stahl, D., J. molec. Evol., (in the press).

  12. 12

    Zablen, L., and Woese, C. R., J. molec. Evol. (in the press).

  13. 13

    Senior, B. W., and Holland, I. B., Proc. natn. Acad. Sci. U.S.A., 68, 959–963 (1971).

  14. 14

    Bowman, C. M., Dahlberg, J. E., Ikemura, T., Konisky, J., and Nomura, M., Proc. natn. Acad. Sci. U.S.A., 68, 964–968 (1971).

  15. 15

    Helser, T. L., Davies, J., and Dahlberg, J. E., Nature new Biol., 235, 6–9 (1972).

  16. 16

    Noller, H. F., Biochemistry, 13, 4694–4703 (1974).

  17. 17

    Santer, M., and Santer, U. V., J. Bact., 116, 1304–1313 (1973).

  18. 18

    Schaup, H. W., Sogin, M., Woese, C., and Kurland, C. G., Molec. gen. Genetics, 114, 1–8 (1971).

  19. 19

    Zimmerman, R. A., Muto, A., Fellner, P., Ehresmann, C., and Branlant, C., Proc. natn. Acad. Sci. U.S.A., 69, 1282–1286 (1972).

  20. 20

    Schaup, H. W., Sogin, M. L., Kurland, C. G., and Woese, C. R., J. Bact., 115, 82–87 (1973).

  21. 21

    Muto, A., Ehresmann, C., Fellner, P., and Zimmermann, R. A., J. molec. Biol., 86, 411–432 (1974).

  22. 22

    Székely, M., Brimacombe, R., and Morgan, J., Eur. J. Biochem., 35, 574–581 (1973).

  23. 23

    Nomura, M., Traub, P., and Bechmann, H., Nature, 219, 793–799 (1968).

  24. 24

    Wrede, P., and Erdmann, V. A., FEBS Letts., 33, 315–319 (1973).

  25. 25

    Gray, P. N., Bellemare, G., Monier, R., Garrett, R. A., and Stöffler, G., J. molec. Biol., 77, 133–152 (1973).

  26. 26

    Fox, G. E., and Woese, C. R., J. molec. Evol., 4, 000 (1974).

  27. 27

    Fellner, P., Ehresmann, C., and Ebel, J. P., Biochimie, 54, 853–900 (1972).

  28. 28

    Santer, M., and Santer, U. V., Biochemistry, 11, 786–791 (1972).

Download references

Author information

Affiliations

  1. Departments of Microbiology and Genetics and Development, University of Illinois, Urbana, Illinois, 61801

    • CARL R. WOESE
    • , GEORGE E. Fox
    • , LAWRENCE ZABLEN
    • , TSUNEKO UCHIDA
    • , LINDA BONEN
    • , KENNETH PECHMAN
    • , BOBBY J. LEWIS
    •  & DAVID STAHL
  2. Mitsubishi-Kasei Institute of Life Sciences, Machida, Tokyo

    • TSUNEKO UCHIDA

Authors

  1. Search for CARL R. WOESE in:

  2. Search for GEORGE E. Fox in:

  3. Search for LAWRENCE ZABLEN in:

  4. Search for TSUNEKO UCHIDA in:

  5. Search for LINDA BONEN in:

  6. Search for KENNETH PECHMAN in:

  7. Search for BOBBY J. LEWIS in:

  8. Search for DAVID STAHL in:

About this article

Publication history

Received

Revised

Issue Date

DOI

https://doi.org/10.1038/254083a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.