Letter | Published:

Improvement of antisera against polypeptide hormones

Naturevolume 254pages8283 (1975) | Download Citation



THE sensitivity of immunoassays is probably decreased by endogenous antigenic material blocking some of the antibody binding sites1. To remove antibodies generated against polypeptide hormones from endogenous antigenic material derived from the immunised animals, we took advantage of the capacity of sodium iodide to break the antigen–antibody bond.

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    Oster, D., Hackenthal, E., Vecsei, P., Gless, K. H., Möhring, J., and Gross, F., in Hypertension (edit. by Distler, A., and Wolff, H-P.) 62 (Georg Thieme, Stuttgart, 1973).

  2. 2

    Freedlender, A. E., Fyhrquist, F., and Hollemans, H. J. G., in Methods of Hormone Radioimmunoassay (edit. by Jaffe, B. M., and Behrman, H. R.) 455 (Academic, New York and London, 1974).

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    Zettner, A., Clin. Chem., 19, 699 (1973).

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    Scatchard, G., Ann. N. Y. Acad. Sci., 51, 660 (1949).

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    Matsukara, S., West, C. D., Ichikawa, Y., Jubiz, G., Harada, G., and Tyler, F. H., J. Lab. clin. Med., 77, 490 (1971).

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    Arimura, A., et al., Endocrinology, 93, 1092 (1973).

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  1. Unit of Clinical Physiology, Minerva Institute for Medical Research, PO Box 819, Helsinki, 10, Finland



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