Letter | Published:

Rapid quantitation of membrane antigens

Naturevolume 254pages6769 (1975) | Download Citation

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Abstract

STUDIES of cell-surface antigens are important in several fields of biological research, notably transplantation and tumour research. The most commonly used tests for such antigens involve assays requiring complements, and the subsequent measurement of cellular damage by the release of radioactive isotopes or the uptake of supravital dyes. Radioimmunoassays which measure directly the antibody–antigen reaction in such studies would have considerable advantages. Although it is possible to label antibody molecules directly, such attempts have so far been unsuccessful because of high background binding values. Furthermore, variations in the composition of the antisera would make each antiserum a unique target for radiolabelling with consequent variations in results. We have tried to circumvent this using a radiolabelled homogeneous marker for IgG molecules produced by certain bacteria.

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References

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    Forsgren, A., and Sjöquist, J., J. Immun., 97, 822 (1966).

  2. 2

    Dorval, G., Welsh, K. I., and Wigzell, H., Scand. J. Immun. (in the press).

  3. 3

    Dausset, J., C.r. Séanc. Soc. Biol. (in the press).

  4. 4

    Welsh, K. I., and Cresswell, P., Transplantation, 12, 234 (1971).

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    Sanderson, A. R., and Welsh, K. I., in Transplantation Antigens, 273 (Academic, London, 1972).

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    Geha, R. S., and Merler, E., Eur. J. Immun., 4, 193 (1974).

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Author information

Affiliations

  1. McIndoe Research Unit, Queen Victoria Hospital, East Grinstead, Sussex, UK

    • K. I. WELSH
  2. Department of Tumour Biology, Karolinska Institute, 104 01, Stockholm, 60

    • G. DORVAL
  3. Department of Immunology, Uppsala University, Box 562, 751 22, Uppsala, 1, Sweden

    • H. WIGZELL

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DOI

https://doi.org/10.1038/254067a0

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