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Direct transformation of 3T3 cells by Abelson murine leukaemia virus


MURINE leukaemia viruses (MLV) cause tumours of haemopoietic origin in vivo1,2, but although they replicate in vitro, they generally do not transform cells. MLV provides helper activity3 for the replication of defective murine sarcoma virus (MSV)4 which possess a transforming activity5. Abelson and Rabstein6 isolated an agent in association with Moloney leukaemia virus (MLV-M) which causes a rapidly progressive lymphoblastic leukaemia of bone-marrow-derived lymphocytes (B cells)7. In addition, this Abelson virus (MLV-A) in conjunction with MLV-M causes rapid appearance of immunoglobulin-producing plasmacytomas in BALB/c mice primed with oil8. We now report that MLV-A can be defective for virus replication and show that this agent directly transforms 3T3 cells in vitro. A quantitative transformation assay is described.

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SCHER, C., SIEGLER, R. Direct transformation of 3T3 cells by Abelson murine leukaemia virus. Nature 253, 729–731 (1975).

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