Abstract
RECONSTITUTION of bacterial ribosomes occurs in vitro in a system containing ribosomal components only1, which suggests that ribosomes also form by self-assembly in vivo. This inference is reinforced by the fact that intermediary particles, containing a similar fraction of ribosomal proteins, are observed both in vitro and in vivo1–3. The in vitro reconstitution process, however, requires a very high energy of activation, presumably to force the intermediates into a rare conformational state1,4. This requirement is too high to be compatible with the rate of ribosome formation in vivo5. Furthermore, there is evidence that the rate-limiting step which leads to the accumulation of the precursor particles in vivo does not involve spontaneous conformational change of these particles6. Although all the information, therefore, required to construct a ribosome must be present in its components, it seems that cells use an additional mechanism to facilitate their assembly.
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References
Nomura, N., Science, 179, 864 (1973).
Mangiarotti, G., Apirion, D., Schlessinger, D., and Silengo, L., Biochemistry, 7, 456 (1968).
Nierhaus, K. H., Bordasch, R., and Homann, H. E., J. molec. Biol., 74, 587 (1973).
Held, W. A., and Nomura, N., Biochemistry, 12, (3273). 1973.
Traub, P., and Nomura, N., J. molec. Biol., 40, 391 (1969).
Turco, E., Altruda, F., Ponzetto, A., and Mangiarotti, G., Biochemistry (in the press).
Adesnik, M., and Levinthal, C., J. molec. Biol., 46, 281 (1969).
Wireman, J. W., and Sypherd, P., Nature, 247, 552 (1974).
Mangiarotti, G., Turco, E., Ponzetto, A., and Altruda, F., Nature, 247, 147 (1974).
Mangiarotti, G., and Turco, E., Eur. J. Biochem., 38, 507 (1973).
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MANGIAROTTI, G., TURCO, E., PERLO, C. et al. Role of precursor 16S RNA in assembly of E. coli 30S ribosomes. Nature 253, 569–571 (1975). https://doi.org/10.1038/253569a0
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DOI: https://doi.org/10.1038/253569a0
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