Abstract
4′-6-DIAMIDINO-2-PHENYLINDOLE (DAPI), which was first synthesised by Dann et al.1 as a trypanocide related to Berenil, has been shown to possess useful DNA binding properties2. Thus, DAPI will bind differentially to yeast mitochondrial and nuclear DNA forming highly fluorescent complexes and enhancing the separation of the two DNAs in caesium chloride gradients2. DAPI can also be used as a highly specific fluorescent stain for both nuclear and mitochondrial DNA in yeast. It seems to be highly sensitive and probably permits detection of a single yeast mitochondrial DNA molecule (D.H.W., and D. J. Fennell, unpublished).
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References
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Williamson, D. H., and Fennell, D. J., in Methods in Cell Biology (edit. by Prescott, D.) (Academic, New York, 1974).
Kenny, G. E., in Contamination in tissue culture (edit. by Fogh J.) (Academic, New York, 1973).
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Hilwig, I., and Gropp, A., Expl Cell. Res., 75, 122 (1972).
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RUSSELL, W., NEWMAN, C. & WILLIAMSON, D. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253, 461–462 (1975). https://doi.org/10.1038/253461a0
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DOI: https://doi.org/10.1038/253461a0
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