Abstract
CHANGES in the number and structure of chromosomes of plant cells cultured in vitro are of common occurrence1, and cell populations devoid of diploid karyotype have been reported2–4. The chromosome number is known to become variable even in clones derived from single cells5,6. In the case of Picea glauca Voss. (n = 21) callus cultures, a positive correlation between growth rate and chromosome number was observed7, which indicates a selection for polyploid cells. Only diploid cells were, however, observed in mitosis in suspension cultures of Haplopappus gracilis (Nutt.) Gray (n = 2) (ref. 2), Medicago sativa L. (n = 16) (ref. 8) and Crepis capillaris L. Wallr. (n = 3) (ref. 9). This could result from polyploid cells dividing less frequently than diploid cells, as diploid and polyploid cells differ physiologically. A strong selection for diploid cells was observed10 in Vicia hajastana Grossh. (n = 5) suspension cultures initiated from mature seeds. It therefore seemed likely that cell cultures of other plant species with a low chromosome number, n = 7 or less, would show a selection for diploid cells.
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SINGH, B., HARVEY, B. Selection for diploid cells in suspension cultures of Haplopappus gracilis. Nature 253, 453 (1975). https://doi.org/10.1038/253453a0
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DOI: https://doi.org/10.1038/253453a0
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