Abstract
IN previous nuclear magnetic resonance (NMR) studies of tRNA we observed anomalously low field resonances (14.5–15.0 p.p.m. downfield from the usual standard 2,2-dimethylsilapentane-5-sulphonate (DSS)) in the spectra of many, but not all, Escherichia coli tRNA1–3 but yeast tRNA we examined never exhibited such resonances1,2. The fact that approximately 60% of the E.coli tRNA are class I and have a 4-thiouridine (S4U) residue at position 8 (refs 4 and 5), whereas yeast tRNA do not, raised the possibility that the anomalous low field resonances in E. coli tRNA might be due to ring NH protons of S4U8 in a tertiary structure base pair.
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WONG, K., KEARNS, D. NMR evidence for tertiary structure base pair in E. coli tRNA involving S4U8. Nature 252, 738–739 (1974). https://doi.org/10.1038/252738a0
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DOI: https://doi.org/10.1038/252738a0
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