Abstract
NEUROBLASTOMA cells in tissue culture have been used widely as an in vitro model for neural functions. Certain ‘cholinergic’ clones have raised choline acetyltransferase activities1, and all clones studied display a high affinity uptake system for exogenous choline2. Whereas low affinity (KT ≃ 10−3 M) choline transport systems occur in various neural and non-neural mammalian tissues, high affinity (KT < 10−5 M), sodium-dependent choline uptake in brain synaptosomes3,4 and the guinea pig intestine (C. B. Pert, and S. H. Snyder, personal communication) is restricted to cholinergic neurones. Efficient conversion of choline to acetylcholine, ionic requirements and sensitivity to inhibitors should uniquely distinguish the cholinergic transport system from those of similar affinity for choline which occur in many cultured mammalian cell lines. We have examined these properties of the choline transport system in cholinergic and non-cholinergic neuroblastoma clones.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Rent or buy this article
Prices vary by article type
from$1.95
to$39.95
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Amano, T., Richelson, E., and Nirenberg, M., Proc. natn. Acad. Sci. U.S.A., 69, 258–263 (1972).
Richelson, E., and Thompson, E. J., Nature, 241, 201–204 (1973).
Haga, T., and Noda, H., Biochim. biophys. Acta., 291, 564–575 (1973).
Yamamura, H. I., and Snyder, S. H., Science, 178, 626–628 (1972).
Blume, A., Gilbert, F., Wilson, S., Farber, J., Rosenberg, R., and Nirengerg, M., Proc. natn. Acad. Sci. U.S.A., 67, 786–792 (1970).
Lanks, K. W., Dorwin, J., and Papirmeister, B., J. Cell Biol. (in the press).
Goldenberg, G. J., Vanstone, C. L., and Bihler, L., Science, 172, 1148–1149 (1971).
Plagemann, P. G., Archs Biochem. Biophys., 128, 70–87 (1968).
Plagemann, P. G. W., J. Lipid Res., 12, 715–724 (1971).
Martin, K., J. Physiol., Lond., 213, 647–664 (1971).
Ellman, G. L., Courtney, K. D., Andres, V., and Featherstone, R. M., Biochem. Pharmac., 7, 88–99 (1961).
McCaman, R. E., and Hunt, J. M., J. Neurochem., 12, 253–259 (1965).
Yagihara, Y., Bleasdale, J. E., and Hawthorne, J. N., J. Neurochem., 21, 173–189 (1973).
Helbronn, E., and Carlsson, B. J., J. Chromatogr., 4, 257–259 (1960).
Harris, A. J., and Dennis, M. J., Science, 167, 1253–1255 (1970).
Simantov, R., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 70, 2902 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LANKS, K., SOMERS, L., PAPIRMEISTER, B. et al. Choline transport by neuroblastoma cells in tissue culture. Nature 252, 476–478 (1974). https://doi.org/10.1038/252476a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/252476a0
This article is cited by
-
PET and PET/CT with radiolabeled choline in prostate cancer: a critical reappraisal of 20 years of clinical studies
European Journal of Nuclear Medicine and Molecular Imaging (2017)
-
The metabolic fate of [3H-methyl]choline in cultured human neuroblastoma cell lines, LA-N-1 and LA-N-2
Molecular and Chemical Neuropathology (1991)
-
Uptake of neurotransmitters and precursors by clonal lines of astrocytoma and neuroblastoma: III. Transport of choline
Neurochemical Research (1976)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.