Abstract
PHOSPHOLIPID vesicles (liposomes1) have been used extensively as models for natural membranes1,2. Multilamellar vesicles are incorporated by mammalian cells both in vivo3,4 and in vitro5. Sonication of such vesicles creates small (250–500 Å diameter) closed spherical vesicles composed of a single lipid bilayer enclosing an aqueous space2. Cultured mammalian cells will also incorporate these unilamellar vesicles without cytotoxic effects6. We present here data on the uptake of unilamellar vesicles by cells in vitro and the use of these vesicles as carriers to introduce biologically-active molecules into specific regions of the cell.
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References
Bangham, A. D., Ann. Rev. Biochem., 41, 753 (1972).
Papahadjopoulos, D., and Kimelberg, H. K., Prog. Surface Sci., 4, 141 (1973).
Gregoriadis, G., FEBS Lett., 36, 292 (1973).
Rahman, Y. -E., Rosenthal, M. W., and Cerny, E. A., Science, 180, 300 (1973).
Gregoriadis, G., and Buckland, G. A., Nature, 244, 170 (1973).
Papahadjopoulos, D., Poste, G., and Mayhew, E., Biochim. biophys. Acta, 363, 404 (1974).
Papahadjopoulos, D., Poste, G., and Schaeffer, B. E., Biochim. biophys. Acta, 323, 23 (1973).
Jung, C. Y., Carlson, L. M., and Baher, C. J., Biochim. biophys. Acta, 298, 101 (1973).
Mayhew, E., and Juliano, R., Expl Cell Res., 77, 409 (1973).
Ehnholm, C., and Zilversmit, D. B., J. biol. Chem., 248, 1719 (1973).
Kamath, S. A., and Rubin, E., Arch. biochem. Biophys., 158, 312 (1973).
Ryser, H. J. P., in Biomembranes (edit. Manson, L.), 2, 197 (Plenum, New York,, 1971).
Sheppard, J. R., in Membranes and viruses in immunopathology (edit. Day, S. B., and Good, R. A.), 249, (Academic Press, New York, 1972).
Papahadjopoulos, D., Poste, G., Schaeffer, B. E., and Vail, W. J., Biochim. biophys. Acta, 352, 10 (1974).
Tappel, A. L., in Lysosomes in biology and pathology (edit. Dingle, J. T., and Fell, H. B.), 2, 207 (North Holland, Amsterdam, 1969).
Inbar, M., and Shinitzky, M., Proc. natn. Acad. Sci. U.S.A., 71, 2128 (1974).
Vail, W. J., Papahadjopoulos, D., and Moscarello, M., Biochim. biophys. Acta, 345, 463 (1974).
Pagano, R. E., Huang, L., and Wey, C., Nature, 252, 166–167 (1974).
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PAPAHADJOPOULOS, D., MAYHEW, E., POSTE, G. et al. Incorporation of lipid vesicles by mammalian cells provides a potential method for modifying cell behaviour. Nature 252, 163–166 (1974). https://doi.org/10.1038/252163a0
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DOI: https://doi.org/10.1038/252163a0
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