Abstract
SEVERAL rapid in vitro methods1–5 for the quantitative assay of murine lymphatic leukaemia viruses (MuLV) have replaced the once standard in vivo bioassay for leukaemogenicity6,7, which was slow, cumbersome, and relatively costly. The in vitro procedures, however, suffer from two limitations: first, some MuLV propagated serially in vitro may lose leukaemogenicity while retaining infectivity in vitro8, so the in vitro assays do not necessarily correlate with biological activity in vivo; second, some wild-type MuLV extracted from normal or neoplastic tissues in vivo seem to require a period of adaptation to in vitro growth conditions before they can be assayed reliably by in vitro methods9.
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DECLEVE, A., LIEBERMAN, M., NIWA, O. et al. Rapid in vivo assay for murine lymphatic leukaemia viruses. Nature 252, 79–81 (1974). https://doi.org/10.1038/252079a0
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DOI: https://doi.org/10.1038/252079a0
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