Abstract
CULTURED human fibroblasts have a limited replicative capacity1 and provide an excellent model for the study of biological ageing1–4. Recent studies have demonstrated that toward the end of their replicative lifespan, normal fibroblasts accumulate a significantly higher proportion of defective enzymes and simultaneously incorporate increased amounts of amino acid analogues5,6. In addition, the RNA base analogue, 5-fluorouracil induces premature senescence of cultured cells which is preceded by the appearance of altered enzyme5. These results have been taken to suggest that the fidelity of protein synthesis decreases, possibly causally, during cellular ageing in vitro5–7.
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GOLDSTEIN, S., SINGAL, D. Alteration of fibroblast gene products in vitro from a subject with Werner's syndrome. Nature 251, 719–721 (1974). https://doi.org/10.1038/251719b0
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DOI: https://doi.org/10.1038/251719b0
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