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Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments

Abstract

Fragments of DNA from derivatives of phage λ having either one or two targets for R.EcoRI have been joined in new combinations to give biologically active phage genomes. These include two classes of deletion mutants; in one, only the DNA between the targets is deleted, but in the second the deletions are more extensive. Other fragments of DNA have been inserted into these phage chromosomes.

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Murray, N., Murray, K. Manipulation of restriction targets in phage λ to form receptor chromosomes for DNA fragments. Nature 251, 476–481 (1974). https://doi.org/10.1038/251476a0

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