Abstract
COLICINOGENIC plasmid E1 (ColE1) has been isolated from Escherichia coli both as a supercoiled DNA molecule of 4.2 × 106 molecular weight and as a supercoiled DNA-protein relaxation complex1. Treatment with certain agents that denature protein structure induces conversion of the super-coiled ColE1 DNA-protein complex to an open circular (relaxed) DNA form with one single strand break specifically in the poly (U,G) binding or ‘Crick’ strand2,3. It has been shown that the EcoRl restriction endonuclease cleaves ColE1 DNA at a single site. Using that site as a reference point it was demonstrated that the single strand break in the open circular product of induced relaxation is located at a unique site, approximately 19% of the length of ColE1 DNA from one end of the EcoRl cleaved molecule4. Only certain restriction endonucleases have previously been shown to cleave DNA at a specific nucleotide sequence and the result of their action is a double strand break5–7.
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LOVETT, M., KATZ, L. & HELINSKI, D. Unidirectional replication of plasmid ColE1 DNA. Nature 251, 337–340 (1974). https://doi.org/10.1038/251337a0
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DOI: https://doi.org/10.1038/251337a0
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