Abstract
WE have recently reported1 the existence of dopamine-sensitive adenyl cyclase (AC) in mouse adrenergic neuroblastoma cells which contain tyrosine hydroxylase (TH) but no choline acetyltransferase (ChA); whereas, nor-adrenaline-sensitive AC at low noradrenaline concentrations becomes apparent in cyclic AMP induced ‘differentiated’ neuroblastoma cells. The presence of acetylcholine receptors in neuroblastoma cells has been demonstrated by Harris and Dennis2. These acetylcholine receptors are blocked by nicotinic and muscarinic inhibitors3. We now report the presence of acetylcholine-sensitive AC in addition to dopamine- and noradrenaline-sensitive AC in inactive neuroblastoma cells which contain neither TH nor ChA, and in cholinergic neuroblastoma cells which contain ChA but no TH. Acetylcholine, dopamine and noradrenaline produced an additive stimulatory effect on AC activity, suggesting that these neurotransmitters act on different receptor sites all of which are linked with AC.
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References
Prasad, K. N., and Gilmer, K. N., Proc. natn. Acad. Sci. U.S.A. (in the press).
Harris, A. J., and Dennis, M. J., Science, 167, 1253 (1970).
Simantov, R., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 70, 2902 (1973).
Prasad, K. N., and Hsie, A. W., Nature new biol., 233, 141 (1971).
Prasad, K. N., Mandal, B., Waymire, J. C., Lees, G. J., Vernadakis, A., and Weiner, N., Nature new Biol., 241, 117 (1973).
Perkins, J. P., and Moore, M. M., J. biol. Chem., 246, 62 (1971).
Krishna, G., Weiss, B., and Brodie, B. B., J. Pharm. exp. Ther., 163, 379 (1968).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J., J. biol. Chem., 193, 265 (1951).
Kebabian, J. W., Petzold, G. L., and Greengard, P., Proc. natn. Acad. Sci. U.S.A., 69, 2145 (1972).
Uzunov, P., and Weiss, B. in Advances in Cyclic Nucleotide Research (edit. by Greengard, P., Robison, G. A. and Paoletti, R.), 1, 435 (Raven Press, New York, 1972).
Guidotti, A., Mao, C. C., and Costa, E., in Proc. third int. Catecholamines Symposium, Strasbourg, France (1973).
Prasad, K. N., Sahu, S. K., and Kumar, S., in Differentiation and Control of Malignancy of Tumor Cells (Gann Monograph on Cancer Research, Tokyo, in the press).
Prasad, K. N., Cancer Res., 31, 1457 (1971).
Prasad, K. N., in The Role of Cyclic Nucleotides in Carcinogenesis (edit. by Gratzner, H., and Schultz, J.), 6, 207 (Academic Press, New York, 1973).
Prasad, K. N., and Kumar, S., in Control of Proliferation in Animal Cells (edit. by Clarkson, B., and Baserga, R.), (Cold Spring Harbor Laboratory, Cold Spring Harbor, in the press).
Sciorelli, G., Poloni, M., and Rindi, G., Brain Res., 48, 427 (1972).
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PRASAD, K., GILMER, K. & SAHU, S. Demonstration of acetylcholine-sensitive adenyl cyclase in malignant neuroblastoma cells in culture. Nature 249, 765–767 (1974). https://doi.org/10.1038/249765a0
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DOI: https://doi.org/10.1038/249765a0
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