Abstract
SEVERAL methods are available for producing synchronous cultures of mammalian cells1,2, but it is technically impossible to produce a culture which maintains a high degree of synchrony for more than one or two rounds of division because of the highly variable length of the period between successive mitoses from cell to cell. Variability of generation time is also observed in sibling cells formed by the division of a common parent. If cells are able to divide accurately siblings would be expected to be genetically and biochemically identical, and should therefore also have identical generation times but in fact while there is a clear correlation3–6 it is always imperfect.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Burk, R. R., Expl. cell Res., 63, 309 (1970).
Terasima, T., and Tolmach, L. S., Nature, 190, 1210 (1961).
Cook, J. E., and Cook, B., Expl. Cell Res., 28, 524 (1962).
Dawson, K. B., Madoc-Jones, H., and Field, E. O., Expl. Cell Res., 38, 75 (1965).
McQuilkin, W. T., and Earle, W. R., J. natn. Cancer Inst., 28, 763 (1962).
Sisken, J. E., and Kinosita, R., Expl. Cell Res., 22, 521 (1961).
Burns, F. J., and Tannock, I. F., Cell Tiss. Kin., 3, 321 (1970).
Smith, J. A., and Martin, L., Proc. natn. Acad. Sci. U.S.A., 70, 1263 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MINOR, P., SMITH, J. Explanation of Degree of Correlation of Sibling Generation Times in Animal Cells. Nature 248, 241–243 (1974). https://doi.org/10.1038/248241a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/248241a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.