Letter | Published:

Loss of methyl acceptor activity of ultraviolet-irradiated tRNA

Naturevolume 247pages561562 (1974) | Download Citation



THE features responsible for the recognition of specific sites for methylation by tRNA methylases are presently not understood. Studies by Kuchino and Nishimura suggest that a specific octanucleotide sequence is recognised by rat liver guanosine methylases1. Furthermore, reports from Nishimura's laboratory show that a large (three-quarter) fragment from E. coli methionine tRNA could be methylated enzymatically2. Shershneva et al.3, however have shown that fragments are not methylated until recombined. Studies in our laboratory have demonstrated that certain fragments from a mixture of E. coli4 tRNAs can serve as substrate for rat liver tRNA methylases. These fragments are believed to have an average S value of 0.64 and therefore would be about ten nucleotides long. Using ultraviolet-induced alterations, we have investigated further the importance of the molecular shape of tRNA as it affects its recognition by tRNA methylases.

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  1. 1

    Kuchino, Y., and Nishimura, S., Biochem. biophys. Res. Comm., 40, 306 (1970).

  2. 2

    Kuchino, Y., Seno, T., and Nishimura, S., Biochem. biophys. Res. Comm. 43, 476 (1971).

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    Shershneva, L. P., Venkstern, T. V., and Bayev, A. A., FEES Lett. 29, 132 (1973).

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    Hancock, R. L., and Dais, D., Int. J. Biochem. (in the press).

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    Hancock, R. L., and Grunau, J., J. Natn. Cancer Inst., 45, 971 (1970).

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    Chaffin, L., Omilianowski, D. R., and Bock, R. M., Science N. Y., 172, 854 (1971).

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    Lipsett, M. N., J. biol. Chem., 240, 3975 (1965).

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  1. Division of Medical Biochemistry, Faculty of Medicine, The University of Calgary, Calgary, 44, Alberta

    •  & R. L. HANCOCK


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