Abstract
THE availability of a large Fourier transform (FT) NMR system in the laboratory of one of us (G. v. B.) allowed us to obtain a series of proton magnetic resonance spectra of nucleosides and dinucleoside monophosphates superior to any hitherto reported. We shall show that the use of the FT-technique at 270 MHz can increase our understanding of the structural features and of the dynamics of the stacking (helix-forming) processes because the chemical shifts as well as the 1H-1H and the 1H-31P coupling constants hidden in the high-field (ribose) part of the spectrum are now in principle amenable to analysis. These parameters provide the key to unlock the secrets of the three-dimensional time-average structure of dinucleosides in solution.
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ALTONA, C., VAN BOOM, J., DE JAGER, J. et al. Conformational Analysis of N6-Methyladenylyl-Uridine. Nature 247, 558–561 (1974). https://doi.org/10.1038/247558a0
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DOI: https://doi.org/10.1038/247558a0
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