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Effects of Cytochalasin B on Uptake and Release of Putative Transmitters by Synaptosomes and on Brain Actomyosin-like Protein

Naturevolume 247pages471473 (1974) | Download Citation

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Abstract

A LARGE variety of contractile cellular functions is thought to be associated with microfilamentous processes which can be disrupted by the fungal metabolite cytochalasin B (ref. 1). To this list of contractile processes have been added the stimulated release of secretory products2–4 as well as transmitter agents5. Based in part on the isolation of actomyosin-like protein (neurostenin) from mammalian brain nerve ending preparations6 a mechanism has been suggested for the exocytotic release of transmitter materials at synaptic endings involving contractile events similar to those occurring during muscle contraction7. We have examined the effect of cytochalasin B on the uptake by rat whole brain nerve ending preparations of noradrenaline, dopamine, glutamic acid and GABA and their K+-stimulated release. We also studied the effect of cytochalasin B on the adenosine triphosphatase activity of the brain actomyosin-like protein and muscle actomyosin.

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Author information

Author notes

    • S. BERL

    Present address: Department of Neurology, Mt Sinai School of Medicine, New York, New York, 10029

Affiliations

  1. Department of Neurology, College of Physicians and Surgeons, 630 W 168th Street, New York, New York, 10032

    • W. J. NICKLAS
    •  & S. BERL

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https://doi.org/10.1038/247471a0

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