Letter | Published:

Increased Mobility and Redistribution of Concanavalin A Receptors on Cells infected with Newcastle Disease Virus

Naturevolume 247pages469471 (1974) | Download Citation

Subjects

Abstract

CONCANAVALIN A (con A) and various other plant lectins have been used extensively to study differences in the organisation of the surface of normal and transformed cells1–3. Cells transformed by oncogenic viruses are agglutinated by doses of con A which do not affect normal cells, but the latter can be made susceptible to agglutination by small doses of con A by brief trypsinisation. Furthermore, the susceptibility of normal cells to agglutination by con A can be increased by infection with various non-oncogenic enveloped viruses, including herpesviruses4,5, poxviruses6, arboviruses7, paramyxoviruses8,9 and rhabdoviruses9. The mechanism of this increased agglutinability is unknown. Since the glycoproteins in the envelope of such viruses can bind con A9–11, their insertion into the plasma membrane of the infected cell during virus envelopment and release might increase the available con A receptors, which could account for the increased susceptibility to agglutination. We have detected no significant increase in con A receptors on cells infected with Newcastle disease virus (NDV) which were susceptible to agglutination by con A at concentrations that did not affect normal uninfected cells. We have found, however, that the increased agglutinability is due to a redistribution of con A receptors on the cell surface.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Lis, H., and Sharon, N., A. Rev. Biochem., 42, 541 (1973).

  2. 2

    Nicolson, G. L., in Control of Proliferation in Animals Cells (edit. by Clarkson, B., and Baserga, R.) (Cold Spring Harbor Laboratory, in the press).

  3. 3

    Burger, M. M., Fed. Proc., 32, 91 (1973).

  4. 4

    Poste, G., Expl. Cell Res., 73, 319 (1972).

  5. 5

    Tevethia, S. S., Lowry, S., Rawls, W. E., Melnick, J. L., and McMillan, V., J. gen. Virol., 15, 93 (1972).

  6. 6

    Zarling, J. M., and Tevethia, S. S., Virology, 45, 313 (1971).

  7. 7

    Birdwell, C. R., and Strauss, J. H., J. Virol., 11, 502 (1973).

  8. 8

    Poste, G., and Reeve, P., Nature new Biol., 237, 113 (1972).

  9. 9

    Rott, R., Becht, H., Klenk, H.-D., and Scholtissek, C., Z. Naturforsch., 27 B, 227 (1972).

  10. 10

    Poste, G., Reeve, P., Alexander, D. J., and Terry, G., J. gen. Virol., 17, 81 (1972).

  11. 11

    Okada, Y., and Kim, J., Virology, 50, 507 (1972).

  12. 12

    Reeve, P., and Poste, G., J. gen. Virol., 11, 17 (1971).

  13. 13

    Reeve, P., Alexander, D. J., Pope, G., and Poste, G., J. gen. Virol., 11, 24 (1971).

  14. 14

    Poste, G., Waterson, A. P., Terry, G., Alexander, D. J., and Terry, G., J. gen. Virol., 16, 95 (1972).

  15. 15

    Reeve, P., Poste, G., Alexander, D. J., and Pope, G., J. gen. Virol., 15, 219 (1972).

  16. 16

    Agrawal, B. B. L., and Goldstein, I. J., Biochim. biophys. Acta, 147, 262 (1967).

  17. 17

    Agrawal, B. B. L., Goldstein, I. J., Hassing, G. S., and So, L. L., Biochemistry, 7, 4211 (1968).

  18. 18

    Reeve, P., Poste, G., and Alexander, D. J., in Negative Strand Viruses (edit. by Barry, R., and Mahy, B. W. J.) (Academic Press, New York, in the press).

  19. 19

    Fox, T. O., Sheppard, J. R., and Burger, M. M., Proc. natn. Acad. Sci. U.S.A., 68, 244 (1971).

  20. 20

    Shoham, J., and Sachs, L., Proc. natn. Acad. Sci. U.S.A., 69, 2479 (1972).

  21. 21

    Nicolson, G. L., Nature new Biol., 243, 218 (1973).

  22. 22

    Rinderknecht, H., Nature, 193, 167 (1962).

  23. 23

    Noonan, K. D., and Burger, M. M., J. biol. Chem., 248, 4286 (1973).

  24. 24

    Rosenblith, J. Z., Ukena, T. E., Yin, H. H., Berlin, R. D., and Karnovsky, M. J., Proc. natn. Acad. Sci. U.S.A., 70, 1625 (1973).

  25. 25

    Imbar, M., and Sachs, L., FEBS Lett., 32, 124 (1973).

  26. 26

    Smith, S. B., and Revel, J.-P., Devl. Biol., 27, 434 (1972).

Download references

Author information

Affiliations

  1. Department of Experimental Pathology, Roswell Park Memorial Institute, Buffalo, New York, 14203

    • G. POSTE
  2. Department of Bacteriology, University College Hospital Medical School, London, WC1E 6JJ

    • P. REEVE

Authors

  1. Search for G. POSTE in:

  2. Search for P. REEVE in:

About this article

Publication history

Received

Revised

Issue Date

DOI

https://doi.org/10.1038/247469a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.