Letter | Published:

Intracellular Location of Specific Antibodies Reacting with Human Lymphocytes

Naturevolume 247pages463465 (1974) | Download Citation

Subjects

Abstract

THE concept of the continuous renewal of the plasma membrane of a cell has been well supported by studies on the rate at which histocompatibility antigens on the surface of the cell are replaced1–4. Thus, if HL-A or H-2 antigens are removed by treatment with proteolytic enzymes, about 6 h elapse before the cell resynthesises them and they are again detectable with the appropriate antisera. When lymphocytes are incubated with a labelled anti-HL-A serum, label is gradually released during the next 6–8 h and the complement-dependent cytotoxic effects of an anti-HL-A serum are lost in a similar way; we call this ‘escape from sensitisation’. This suggests that the turnover of the membrane of a cell may be primarily responsible for the ability of the cell to deal with antibodies binding to it, but the mechanisms involved are by no means clear. It has been suggested that antibody could either be released from the surface of the cell as an antigen-antibody complex or that it might be internalised, catabolised and then released, but neither of these ideas has been fully investigated. The early fate of fluorescent material has however been extensively studied5,6,7.

References

  1. 1

    Turner, M. J., Strominger, J. L., and Sanderson, A. R., Proc. natn. Acad. Sci. U.S.A., 69, 200 (1972).

  2. 2

    Schwartz, B. D., and Nathenson, S. G., Transplantn. Proc., 3, 180 (1971).

  3. 3

    Chapel, H. M., and Welsh, K. P., Transplantation, 13, 347 (1972).

  4. 4

    Miyajima, T., Hirata, A. A., and Terasaki, P. I., Tis. Antigens, 2, 64 (1972).

  5. 5

    Taylor, R. B., Duffus, P. H., Raff, M. C., and De Petris, S., Nature new Biol., 233, 225 (1971).

  6. 6

    De Petris, S., and Raff, M. C., Eur. J. Immun., 2, 523 (1972).

  7. 7

    Menne, H. D., and Fladd, H. D., Clin. exp. Immun., 14, 57 (1973).

  8. 8

    Dumonde, D. C., Al-Askari, S., Lawrence, H. S., and Thomas, L., Nature, 198, 598 (1963).

  9. 9

    Herberman, R., and Stetson, C. A., J. exp. Med., 121, 533 (1965).

  10. 10

    Billingham, R. E., Brent, L., and Medawar, P. B., Nature, 178, 512 (1956).

  11. 11

    Albert, W. H. W., and Davies, D. A. L., Immunology, 24, 841 (1973).

  12. 12

    Hunter, W. M., and Greenwood, F. C., Nature, 194, 495 (1962).

  13. 13

    Pegrum, G. D., Perera, D. J. B., and Thompson, E., Immunology, 23, 13 (1972).

  14. 14

    Michalowski, A., Jasinska, J., Brzosko, W. J., and Nowalowski, A., Expl. cell Res., 34, 417 (1964).

  15. 15

    Stanley, D. A., Frenster, J. H., and Riggs, D. A., Int. Proc. 4th Leuk. Cult. Conf. (edit. by McIntyre, O. R.) (Meredith, New York, 1971).

  16. 16

    Lewis, C. M., and Pegrum, G. D., Immunology, 24, 1013 (1973).

  17. 17

    Greaves, M. F., and Bauminger, S., Nature, 235, 237 (1972).

  18. 18

    Andersson, J., Edelman, G. M., Moller, G., and Sjoberg, O., Eur. J. Immun., 2, 233 (1972).

Download references

Author information

Affiliations

  1. Haematology Department, Charing Cross Hospital (Fulham), Fulham Palace Road, Hammersmith, London, W6

    • C. M. LEWIS
    • , G. D. PEGRUM
    •  & CAROL A. EVANS

Authors

  1. Search for C. M. LEWIS in:

  2. Search for G. D. PEGRUM in:

  3. Search for CAROL A. EVANS in:

About this article

Publication history

Received

Issue Date

DOI

https://doi.org/10.1038/247463a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.