Letter | Published:

Vaccination of Rhesus monkeys against Malaria by use of sucrose density gradient fractions of Plasmodium knowlesi antigens

Naturevolume 247pages304305 (1974) | Download Citation



WE wish to report the results of immunity induction trials in the Plasmodium knowlesi-Rhesus monkey system. Thirteen juvenile (1.5–2.0 kg) Rhesus monkeys (Macaca mulatta) were vaccinated with various non-viable fractionation products derived from the plasmodial parasite, P. knowlesi. Samples were prepared from parasitised erythrocytes by differential French Pressure Cell disintegration and centrifugation, Bio-Gel column chromatographic separation, and linear sucrose density gradient fractionation. Two injections of the respective antigen preparations, emulsified in Freund's adjuvant, were given at 4-week intervals, followed 4 weeks later by blood-stage challenge with the homologous P. knowlesi strain. The resulting data reflect a significant difference in peak parasitaemias of the vaccinated monkeys as compared to control monkeys.

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    Present address: Rush Medical College, Chicago, Illinois


  1. Department of Biology, University of New Mexico Albuquerque, New Mexico, 87131



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