Abstract
WE wish to report the results of immunity induction trials in the Plasmodium knowlesi-Rhesus monkey system. Thirteen juvenile (1.5–2.0 kg) Rhesus monkeys (Macaca mulatta) were vaccinated with various non-viable fractionation products derived from the plasmodial parasite, P. knowlesi. Samples were prepared from parasitised erythrocytes by differential French Pressure Cell disintegration and centrifugation, Bio-Gel column chromatographic separation, and linear sucrose density gradient fractionation. Two injections of the respective antigen preparations, emulsified in Freund's adjuvant, were given at 4-week intervals, followed 4 weeks later by blood-stage challenge with the homologous P. knowlesi strain. The resulting data reflect a significant difference in peak parasitaemias of the vaccinated monkeys as compared to control monkeys.
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SIMPSON, G., SCHENKEL, R. & SILVERMAN, P. Vaccination of Rhesus monkeys against Malaria by use of sucrose density gradient fractions of Plasmodium knowlesi antigens. Nature 247, 304–305 (1974). https://doi.org/10.1038/247304a0
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DOI: https://doi.org/10.1038/247304a0
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