Abstract
CHROMOSOME banding methods have been widely used to identify normal and rearranged chromosomes1–11 and to probe the structural and biochemical organisation of chromosomes11–13. The methods most commonly used include Q1,2, G3–6, C7 and R banding8, each producing characteristic bands on metaphase chromosomes as observed by light microscopy. Attempts to observe banding at an ultrastructural level have not been particularly successful12,14,15, but since such studies could provide more detailed maps of chromosomes and help to clarify the structural nature of the bands, further attempts were made in my laboratory to develop a reliable and simple method for observing chromosome bands at the electron microscope level, using trypsin as the banding agent5.
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BURKHOLDER, G. Electron Microscopic Visualisation of Chromosomes banded with Trypsin. Nature 247, 292–294 (1974). https://doi.org/10.1038/247292a0
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DOI: https://doi.org/10.1038/247292a0
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