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Electron Microscopic Visualisation of Chromosomes banded with Trypsin

Naturevolume 247pages292294 (1974) | Download Citation

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Abstract

CHROMOSOME banding methods have been widely used to identify normal and rearranged chromosomes1–11 and to probe the structural and biochemical organisation of chromosomes11–13. The methods most commonly used include Q1,2, G3–6, C7 and R banding8, each producing characteristic bands on metaphase chromosomes as observed by light microscopy. Attempts to observe banding at an ultrastructural level have not been particularly successful12,14,15, but since such studies could provide more detailed maps of chromosomes and help to clarify the structural nature of the bands, further attempts were made in my laboratory to develop a reliable and simple method for observing chromosome bands at the electron microscope level, using trypsin as the banding agent5.

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References

  1. 1

    Caspersson, T., Zech, L., Wagh, U., Modest, E. J., and Simonsson, E., Expl. Cell Res., 58, 141 (1969).

  2. 2

    Caspersson, T., Zech, L., Johansson, C., and Modest, E. J., Chromosoma, 30, 215 (1970).

  3. 3

    Sumner, A. T., Evans, H. J., and Buckland, R. A., Nature new Biol., 232, 31 (1971).

  4. 4

    Drets, M. E., and Shaw, M. W., Proc. natn. Acad. Sci. U.S.A., 68, 2073 (1971).

  5. 5

    Wang, H. C., and Fedoroff, S., Nature new Biol., 235, 52 (1972).

  6. 6

    Kato, H., and Yosida, T. H., Chromosoma, 36, 272 (1972).

  7. 7

    Arrighi, F. E., and Hsu, T. C., Cytogenetics, 10, 81 (1971).

  8. 8

    Dutrillaux, B., and Lejeune, J., Cr. hebd. Séanc. Acad. Sci. Paris, 272, 2638 (1971).

  9. 9

    Miller, D. A., Allderdice, P. W., Miller, O. J., and Breg, W. R., Nature, 232, 24 (1971).

  10. 10

    Francke, U., Am. J. human Genet., 24, 189 (1972).

  11. 11

    Caspersson, T., and Zech, L. (eds.), Chromosome Identification—Techniques and Applications in Biology and Medicine (Academic Press, New York, 1973).

  12. 12

    Comings, D. E., Avelino, E., Okada, T. A., and Wyandt, H. E., Expl Cell Res., 77, 469 (1973).

  13. 13

    Schreck, R. R., Warburton, D., Miller, O. J., Beiser, S. M., and Erlanger, B. F., Proc. natn. Acad. Sci. U.S.A., 70, 804 (1973).

  14. 14

    Bahr, G. F., Mikel, U., and Engler, W. J., in Chromosome Identification—Techniques and Applications in Biology and Medicine (edit. by Caspersson, T., and Zech, L.), 280 (Academic Press, New York, 1973).

  15. 15

    Ridler, M. A. C., and Ohara, P. T., Lancet, i, 1339 (1972).

  16. 16

    DuPraw, E. J., Nature, 206, 338 (1965).

  17. 17

    Comings, D. E., and Okada, T. A., in Perspectives in Cytogenetics (edit. by Wright, S. W., Crandall, B. F., and Boyer, L.), 223 (Thomas, Springfield, Illinois, 1972).

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  1. Department of Anatomy, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 0WO

    • GARY D. BURKHOLDER

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https://doi.org/10.1038/247292a0

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