Abstract
IN the course of studies of the metabolism of intracerebrally administered L-U-14C-histidine in brains of mice and frogs (Rana pipiens), analyses were also performed on blood samples from the injected animals. The mice received 1.5×106 c.p.m. of the labelled histidine, and 1 h later samples of blood were taken by means of orbital puncture. Frogs were injected with 2.9×106 c.p.m. and blood was obtained after 1 h by cardiac puncture. The samples were deproteinised in 5 volumes of 0.4 N perchloric acid and processed in the manner described previously for brain and tumour tissues1. Aliquots of neutralised extracts were electrophoresed on Whatman No. 3 paper at pH 10.0 together with known standards of carnosine, homocarnosine, histidine, imidazoleacetic acid, N-acetylhistidine, and histamine; and stained with Fast Red B in the manner described previously1.
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References
Van Balgooy, J. N. A., Marshall, F. D., and Roberts, E., J. Neurochem., 19, 2341 (1972).
Van Balgooy, J. N. A., and Roberts, E., Biochem. Pharmac., 22, 1405 (1973).
Gulewitsch, W., and Amiradzibi, S., Ber. dt. chem. Ges., 33, 1902 (1900).
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BALGOOY, J., MARSHALL, F. & ROBERTS, E. Carnosine in Nucleated Erythrocytes. Nature 247, 226–227 (1974). https://doi.org/10.1038/247226a0
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DOI: https://doi.org/10.1038/247226a0
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